Antibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 12-1159-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD115 (c-fms) Monoclonal Antibody (12-3A3-1B10), PE, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: CD115 or Colony-Stimulating Factor-1 Receptor (CSF-1R) is expressed on cells committed to the monocyte lineage and osteoclasts. CSF-1R is a disulfide-linked homodimer, which upon binding of its ligand, CSF-1, undergoes tyrosine autophosphorylation and subsequently, phosphorylates other membrane-proximal downstream targets which results in cytoskeletal remodeling, gene transcription and protein translation. CSF-1R activation promotes the survival, proliferation and differentiation of mononuclear phagocytes and the spreading and motility of macrophages. In osteoclasts, CSF-1R synergizes with RANKL to regulate the differentiation of mononuclear phagocytes to osteoclasts. Applications Reported: This 12-3A3-1B10 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 12-3A3-1B10 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood. This can be used at 5 µL (0.03 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Rat
- Conjugate
- Yellow dye
- Isotype
- IgG
- Antibody clone number
- 12-3A3-1B10
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Macrophage-Colony-Stimulating Factor Receptor Enhances Prostate Cancer Cell Growth and Aggressiveness In Vitro and In Vivo and Increases Osteopontin Expression.
BCG Vaccination in Humans Elicits Trained Immunity via the Hematopoietic Progenitor Compartment.
Human lactoferrin attenuates the proinflammatory response of neonatal monocyte-derived macrophages.
Circulating precursors of human CD1c+ and CD141+ dendritic cells.
In vivo pre-activation of monocytes in patients with axial spondyloarthritis.
Characterization of Gaucher disease bone marrow mesenchymal stromal cells reveals an altered inflammatory secretome.
Plasticity of dendritic cell function in response to prostaglandin E2 (PGE2) and interferon-gamma (IFN-gamma).
Monoclonal antibodies to the human CSF-1 receptor (c-fms proto-oncogene product) detect epitopes on normal mononuclear phagocytes and on human myeloid leukemic blast cells.
Mougel A, Adriaenssens E, Guyot B, Tian L, Gobert S, Chassat T, Persoons P, Hannebique D, Bauderlique-Le Roy H, Vicogne J, Le Bourhis X, Bourette RP
International journal of molecular sciences 2022 Dec 16;23(24)
International journal of molecular sciences 2022 Dec 16;23(24)
BCG Vaccination in Humans Elicits Trained Immunity via the Hematopoietic Progenitor Compartment.
Cirovic B, de Bree LCJ, Groh L, Blok BA, Chan J, van der Velden WJFM, Bremmers MEJ, van Crevel R, Händler K, Picelli S, Schulte-Schrepping J, Klee K, Oosting M, Koeken VACM, van Ingen J, Li Y, Benn CS, Schultze JL, Joosten LAB, Curtis N, Netea MG, Schlitzer A
Cell host & microbe 2020 Aug 12;28(2):322-334.e5
Cell host & microbe 2020 Aug 12;28(2):322-334.e5
Human lactoferrin attenuates the proinflammatory response of neonatal monocyte-derived macrophages.
Wisgrill L, Wessely I, Spittler A, Förster-Waldl E, Berger A, Sadeghi K
Clinical and experimental immunology 2018 Jun;192(3):315-324
Clinical and experimental immunology 2018 Jun;192(3):315-324
Circulating precursors of human CD1c+ and CD141+ dendritic cells.
Breton G, Lee J, Zhou YJ, Schreiber JJ, Keler T, Puhr S, Anandasabapathy N, Schlesinger S, Caskey M, Liu K, Nussenzweig MC
The Journal of experimental medicine 2015 Mar 9;212(3):401-13
The Journal of experimental medicine 2015 Mar 9;212(3):401-13
In vivo pre-activation of monocytes in patients with axial spondyloarthritis.
Conrad K, Wu P, Sieper J, Syrbe U
Arthritis research & therapy 2015 Jul 16;17(1):179
Arthritis research & therapy 2015 Jul 16;17(1):179
Characterization of Gaucher disease bone marrow mesenchymal stromal cells reveals an altered inflammatory secretome.
Campeau PM, Rafei M, Boivin MN, Sun Y, Grabowski GA, Galipeau J
Blood 2009 Oct 8;114(15):3181-90
Blood 2009 Oct 8;114(15):3181-90
Plasticity of dendritic cell function in response to prostaglandin E2 (PGE2) and interferon-gamma (IFN-gamma).
Lehner M, Stilper A, Morhart P, Holter W
Journal of leukocyte biology 2008 Apr;83(4):883-93
Journal of leukocyte biology 2008 Apr;83(4):883-93
Monoclonal antibodies to the human CSF-1 receptor (c-fms proto-oncogene product) detect epitopes on normal mononuclear phagocytes and on human myeloid leukemic blast cells.
Ashmun RA, Look AT, Roberts WM, Roussel MF, Seremetis S, Ohtsuka M, Sherr CJ
Blood 1989 Feb 15;73(3):827-37
Blood 1989 Feb 15;73(3):827-37
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Rat IgG2a Isotype Contol PE (Product # 12-4321-80) (open histogram) or Anti-Human CD115 (c-fms) PE (filled histogram). Cells in the monocyte gate were used for analysis.
- Conjugate
- Yellow dye