Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- 13-1159-80 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD115 (c-fms) Monoclonal Antibody (12-3A3-1B10), Biotin, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: CD115 or Colony-Stimulating Factor-1 Receptor (CSF-1R) is expressed on cells committed to the monocyte lineage and osteoclasts. CSF-1R is a disulfide-linked homodimer, which upon binding of its ligand, CSF-1, undergoes tyrosine autophosphorylation and subsequently, phosphorylates other membrane-proximal downstream targets which results in cytoskeletal remodeling, gene transcription and protein translation. CSF-1R activation promotes the survival, proliferation and differentiation of mononuclear phagocytes and the spreading and motility of macrophages. In osteoclasts, CSF-1R synergizes with RANKL to regulate the differentiation of mononuclear phagocytes to osteoclasts.
- Conjugate
- Biotin
- Antibody clone number
- 12-3A3-1B10
- Concentration
- 0.5 mg/mL
Submitted references Monoclonal antibodies to the human CSF-1 receptor (c-fms proto-oncogene product) detect epitopes on normal mononuclear phagocytes and on human myeloid leukemic blast cells.
Ashmun RA, Look AT, Roberts WM, Roussel MF, Seremetis S, Ohtsuka M, Sherr CJ
Blood 1989 Feb 15;73(3):827-37
Blood 1989 Feb 15;73(3):827-37
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Staining of normal human peripheral blood cells with 0.06 µg of Rat IgG2b K Isotype Control Biotin (Product # 13-4321-82) (open histogram) or 0.06 µg of Anti-Human CD115 (c-fms) Biotin (filled histogram) followed by Streptavidin PE (Product # 12-4317-87). Cells in the monocyte gate were used for analysis.
- Conjugate
- Biotin