Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- 25-1159-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD115 (c-fms) Monoclonal Antibody (12-3A3-1B10), PE-Cyanine7, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: CD115 or Colony-Stimulating Factor-1 Receptor (CSF-1R) is expressed on cells committed to the monocyte lineage and osteoclasts. CSF-1R is a disulfide-linked homodimer, which upon binding of its ligand, CSF-1, undergoes tyrosine autophosphorylation and subsequently, phosphorylates other membrane-proximal downstream targets which results in cytoskeletal remodeling, gene transcription and protein translation. CSF-1R activation promotes the survival, proliferation and differentiation of mononuclear phagocytes and the spreading and motility of macrophages. In osteoclasts, CSF-1R synergizes with RANKL to regulate the differentiation of mononuclear phagocytes to osteoclasts. Applications Reported: This 12-3A3-1B10 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 12-3A3-1B10 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.03 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Rat
- Isotype
- IgG
- Antibody clone number
- 12-3A3-1B10
- Vial size
- 100 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Monoclonal antibodies to the human CSF-1 receptor (c-fms proto-oncogene product) detect epitopes on normal mononuclear phagocytes and on human myeloid leukemic blast cells.
Ashmun RA, Look AT, Roberts WM, Roussel MF, Seremetis S, Ohtsuka M, Sherr CJ
Blood 1989 Feb 15;73(3):827-37
Blood 1989 Feb 15;73(3):827-37
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD11b APC (Product # 17-0118-42) and Rat IgG2a K Isotype Control PE-Cyanine7 (Product # 25-4321-82) (left) or Anti-Human CD115 (c-fms) PE-Cyanine7 (right). Cells in the lymphocyte and monocyte gate were used for analysis.