Antibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- 14-1159-37 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD115 (c-fms) Monoclonal Antibody (12-3A3-1B10), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: CD115 or Colony-Stimulating Factor-1 Receptor (CSF-1R) is expressed on cells committed to the monocyte lineage and osteoclasts. CSF-1R is a disulfide-linked homodimer, which upon binding of its ligand, CSF-1, undergoes tyrosine autophosphorylation and subsequently, phosphorylates other membrane-proximal downstream targets which results in cytoskeletal remodeling, gene transcription and protein translation. CSF-1R activation promotes the survival, proliferation and differentiation of mononuclear phagocytes and the spreading and motility of macrophages. In osteoclasts, CSF-1R synergizes with RANKL to regulate the differentiation of mononuclear phagocytes to osteoclasts.
- Antibody clone number
- 12-3A3-1B10
- Concentration
- 0.5 mg/mL
Submitted references A unique anti-CD115 monoclonal antibody which inhibits osteolysis and skews human monocyte differentiation from M2-polarized macrophages toward dendritic cells.
Comparison of gene expression profiles between human and mouse monocyte subsets.
Haegel H, Thioudellet C, Hallet R, Geist M, Menguy T, Le Pogam F, Marchand JB, Toh ML, Duong V, Calcei A, Settelen N, Preville X, Hennequi M, Grellier B, Ancian P, Rissanen J, Clayette P, Guillen C, Rooke R, Bonnefoy JY
mAbs 2013 Sep-Oct;5(5):736-47
mAbs 2013 Sep-Oct;5(5):736-47
Comparison of gene expression profiles between human and mouse monocyte subsets.
Ingersoll MA, Spanbroek R, Lottaz C, Gautier EL, Frankenberger M, Hoffmann R, Lang R, Haniffa M, Collin M, Tacke F, Habenicht AJ, Ziegler-Heitbrock L, Randolph GJ
Blood 2010 Jan 21;115(3):e10-9
Blood 2010 Jan 21;115(3):e10-9
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Staining of normal human peripheral blood cells with 0.5 µg of Rat IgG2a K Isotype Control Purified (Product # 14-4321-82) (open histogram) or 0.5 µg of Anti-Human CD115 (c-fms) Purified (filled histogram) followed by Anti-Rat IgG Biotin (Product # 13-4813-85) and Streptavidin PE (Product # 12-4317-87). Cells in the monocyte gate were used for analysis.