PA5-25974

antibody from Invitrogen Antibodies

Targeting: CSF1R C-FMS, CD115, CSFR, FMS

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-25974

Provider

Invitrogen Antibodies

Product name

CSF1R Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µL

Concentration

1.8 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Minocycline suppresses disease-associated microglia (DAM) in a model of photoreceptor cell degeneration.

Ozaki E, Delaney C, Campbell M, Doyle SL
Experimental eye research 2022 Apr;217:108953

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Evidence of a Myenteric Plexus Barrier and Its Macrophage-Dependent Degradation During Murine Colitis: Implications in Enteric Neuroinflammation.

Dora D, Ferenczi S, Stavely R, Toth VE, Varga ZV, Kovacs T, Bodi I, Hotta R, Kovacs KJ, Goldstein AM, Nagy N
Cellular and molecular gastroenterology and hepatology 2021;12(5):1617-1641

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells using a CSF1R polyclonal antibody (Product # PA5-25974) at a dilution of 1:10-50, followed by a fluor-conjugated goat anti-rabbit secondary antibody (green). Nuclei were stained with DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of CSF1R was performed using 70% confluent log phase THP-1 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with anti-CSF1R Polyclonal Antibody (Product # PA5-25974) at 1:200 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing Plasma Membrane localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of K562 cells using a CD115/MCSF Receptor polyclonal antibody (Product # PA5-25974) (bottom) compared to a negative control cell (top) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 2 Immunophenotype and spatial distribution of IGMs in CX3CR1 GFP mouse colon. GFP+ IGMs are present within the agrin-bordered enteric ganglia and co-express CD11b ( A-A"" ), CSF1R ( B-B"" ), F4/80 ( C-C"" ), and Iba1 ( D-D"" ). F4/80 and Iba1 are co-expressed by MMs and IGMs ( F-F"" , arrowheads , magnified from circled area in E ), but Iba1 is not present in submucosal macrophages ( F-F"" , arrows ). In lymphatic aggregates of the colonic mucosa ( E , area within dashed line ), macrophages express Iba1 but not F4/80. Comparison of MM and IGM cell number in the proximal and distal colon of CX3CR1 GFP mice shows no significant difference in ratio of IGMs to total MMs ( G ; 3.26 % +- 1.3 % vs 2.12 % +- 1.1 %, P = .0977, n = 8). However, the number of IGMs is significantly higher in distal colon compared with proximal colon when adjusted to myenteric ganglion area ( H ; 82.37 +- 32.98 vs 50.25 +- 19.6 cell/mm 2 , P = .0309, n = 8).