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LearnPA5-103797
antibody from Invitrogen Antibodies
Targeting: POLR1E
FLJ13390, FLJ13970, PAF53, PRAF1, RPA49
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- PA5-103797 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- POLR1E Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Antibody detects endogenous levels of total RPA49.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of POLR1E in K562 cell lysate. Samples were incubated with POLR1E polyclonal antibody (Product # PA5-103797).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of POLR1E in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with POLR1E polyclonal antibody (Product # PA5-103797) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.
