A304-835A-T

antibody from Invitrogen Antibodies

Targeting: POLR1E FLJ13390, FLJ13970, PAF53, PRAF1, RPA49

Western blot (Recommended by provider)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A304-835A-T

Provider

Invitrogen Antibodies

Product name

POLR1E/PRAF1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

10 µL

Concentration

1 mg/mL

Storage

4° C

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human POLR1E/PRAF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-POLR1E/PRAF1 antibody A304-835A (lot A304-835A-1) used for IP at 6 µg per reaction. POLR1E/PRAF1 was also immunoprecipitated by rabbit anti-POLR1E/PRAF1 antibody A304-834A. For blotting immunoprecipitated POLR1E/PRAF1, A304-835A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.