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Western blot
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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- R33928 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- PHLDA2 Antibody / Pleckstrin homology-like domain, family A, member 2
- Antibody type
- Polyclonal
- Description
- This highly specific PHLDA2 antibody is suitable for use in Immunohistochemistry/Western blot/Immunofluorescence/Flow cytometry/ELISA applications with human samples.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide
- Storage
- Aliquot and store the PHLDA2 antibody at -20oC.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human MDA-MB-231 cell lysate with PHLDA2 antibody. Predicted molecular weight ~17 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IF/ICC staining of fixed and permeabilized human HeLa cells with PHLDA2 antibody (green) at 10ug/ml and DAPI nuclear stain (blue).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human placenta PHLDA2 antibody at 4ug/ml. HIER: steamed with pH6 citrate buffer, AP-staining.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human prostate tissue with PHLDA2 antibody at 4ug/ml. HIER: steamed with pH6 citrate buffer, AP-staining.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- FACS testing of fixed and permeabilized human MCF7 cells with PHLDA2 antibody (blue) at 10ug/ml and naive goat Ig (black).
