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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- LS-C287104 - Provider product page
- Provider
- LSBio
- Product name
- ATF7IP / MCAF1 Antibody (aa25-100) LS-C287104
- Antibody type
- Polyclonal
- Description
- Affinity purified
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Store at 2-8°C for up to one year.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Detection of Human MCAF by Western Blot. Samples: Nuclear extract (20 ug) from HeLa cells. Antibody: Affinity purified rabbit anti-MCAF antibody used at 0.2 and 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 15 minutes.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Detection of human MCAF by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MCAF antibody used for WB at 0.05 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Detection of Human MCAF by Immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-MCAF used at a dilution of 1:1000 (1 Detection: DAB.
