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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- A300-169A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MCAF Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human MCAF by western blot. Samples: Whole cell lysate (50 µg) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MCAF antibody A300-169A (lot A300-169A-1) used for WB at 0.05 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human MCAF by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-MCAF (Cat. No. A300-169A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.
