Antibody data
- Antibody Data
- Antigen structure
- References [14]
- Comments [0]
- Validations
- Western blot [5]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- GTX110527 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX110527, RRID:AB_10721789
- Product name
- IL6 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
Submitted references M2 macrophage-derived IL6 mediates resistance of breast cancer cells to hedgehog inhibition.
In vitro studies on anti-inflammatory activities of kiwifruit peel extract in human THP-1 monocytes.
17β-estradiol upregulates IL6 expression through the ERβ pathway to promote lung adenocarcinoma progression.
Vascular niche IL-6 induces alternative macrophage activation in glioblastoma through HIF-2α.
Cytokine expression associated with Helicobacter pylori and Epstein-Barr virus infection in gastric carcinogenesis.
Fisetin Regulates Nrf2 Expression and the Inflammation-Related Signaling Pathway to Prevent UVB-Induced Skin Damage in Hairless Mice.
A standardized herbal extract mitigates tumor inflammation and augments chemotherapy effect of docetaxel in prostate cancer.
Therapeutic effect of urine-derived stem cells for protamine/lipopolysaccharide-induced interstitial cystitis in a rat model.
IL-5 and IL-6 are increased in the frontal recess of eosinophilic chronic rhinosinusitis patients.
Role of Expression of Inflammatory Mediators in Primary and Recurrent Lumbar Disc Herniation.
Secretory RAB GTPase 3C modulates IL6-STAT3 pathway to promote colon cancer metastasis and is associated with poor prognosis.
Cyclophosphamide-induced HCN1 channel upregulation in interstitial Cajal-like cells leads to bladder hyperactivity in mice.
Chitosan Treatment Delays the Induction of Senescence in Human Foreskin Fibroblast Strains.
Anti-inflammatory Effects of Resveratrol on Hypoxia/Reoxygenation-Induced Alveolar Epithelial Cell Dysfunction.
Xu X, Ye J, Huang C, Yan Y, Li J
Toxicology and applied pharmacology 2019 Feb 1;364:77-82
Toxicology and applied pharmacology 2019 Feb 1;364:77-82
In vitro studies on anti-inflammatory activities of kiwifruit peel extract in human THP-1 monocytes.
D'Eliseo D, Pannucci E, Bernini R, Campo M, Romani A, Santi L, Velotti F
Journal of ethnopharmacology 2019 Apr 6;233:41-46
Journal of ethnopharmacology 2019 Apr 6;233:41-46
17β-estradiol upregulates IL6 expression through the ERβ pathway to promote lung adenocarcinoma progression.
Huang Q, Zhang Z, Liao Y, Liu C, Fan S, Wei X, Ai B, Xiong J
Journal of experimental & clinical cancer research : CR 2018 Jul 3;37(1):133
Journal of experimental & clinical cancer research : CR 2018 Jul 3;37(1):133
Vascular niche IL-6 induces alternative macrophage activation in glioblastoma through HIF-2α.
Wang Q, He Z, Huang M, Liu T, Wang Y, Xu H, Duan H, Ma P, Zhang L, Zamvil SS, Hidalgo J, Zhang Z, O'Rourke DM, Dahmane N, Brem S, Mou Y, Gong Y, Fan Y
Nature communications 2018 Feb 8;9(1):559
Nature communications 2018 Feb 8;9(1):559
Cytokine expression associated with Helicobacter pylori and Epstein-Barr virus infection in gastric carcinogenesis.
Kim JY, Bae BN, Kang G, Kim HJ, Park K
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 2017 Sep;125(9):808-815
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 2017 Sep;125(9):808-815
Fisetin Regulates Nrf2 Expression and the Inflammation-Related Signaling Pathway to Prevent UVB-Induced Skin Damage in Hairless Mice.
Wu PY, Lyu JL, Liu YJ, Chien TY, Hsu HC, Wen KC, Chiang HM
International journal of molecular sciences 2017 Oct 10;18(10)
International journal of molecular sciences 2017 Oct 10;18(10)
A standardized herbal extract mitigates tumor inflammation and augments chemotherapy effect of docetaxel in prostate cancer.
Tsai CH, Tzeng SF, Hsieh SC, Yang YC, Hsiao YW, Tsai MH, Hsiao PW
Scientific reports 2017 Nov 15;7(1):15624
Scientific reports 2017 Nov 15;7(1):15624
Therapeutic effect of urine-derived stem cells for protamine/lipopolysaccharide-induced interstitial cystitis in a rat model.
Li J, Luo H, Dong X, Liu Q, Wu C, Zhang T, Hu X, Zhang Y, Song B, Li L
Stem cell research & therapy 2017 May 8;8(1):107
Stem cell research & therapy 2017 May 8;8(1):107
IL-5 and IL-6 are increased in the frontal recess of eosinophilic chronic rhinosinusitis patients.
Kubota K, Takeno S, Taruya T, Sasaki A, Ishino T, Hirakawa K
Journal of otolaryngology - head & neck surgery = Le Journal d'oto-rhino-laryngologie et de chirurgie cervico-faciale 2017 May 2;46(1):36
Journal of otolaryngology - head & neck surgery = Le Journal d'oto-rhino-laryngologie et de chirurgie cervico-faciale 2017 May 2;46(1):36
Role of Expression of Inflammatory Mediators in Primary and Recurrent Lumbar Disc Herniation.
Dagistan Y, Cukur S, Dagistan E, Gezici AR
Journal of Korean Neurosurgical Society 2017 Jan 1;60(1):40-46
Journal of Korean Neurosurgical Society 2017 Jan 1;60(1):40-46
Secretory RAB GTPase 3C modulates IL6-STAT3 pathway to promote colon cancer metastasis and is associated with poor prognosis.
Chang YC, Su CY, Chen MH, Chen WS, Chen CL, Hsiao M
Molecular cancer 2017 Aug 7;16(1):135
Molecular cancer 2017 Aug 7;16(1):135
Cyclophosphamide-induced HCN1 channel upregulation in interstitial Cajal-like cells leads to bladder hyperactivity in mice.
Liu Q, Long Z, Dong X, Zhang T, Zhao J, Sun B, Zhu J, Li J, Wang Q, Yang Z, Hu X, Li L
Experimental & molecular medicine 2017 Apr 21;49(4):e319
Experimental & molecular medicine 2017 Apr 21;49(4):e319
Chitosan Treatment Delays the Induction of Senescence in Human Foreskin Fibroblast Strains.
Tsai CW, Kao YT, Chiang IN, Wang JH, Young TH
PloS one 2015;10(10):e0140747
PloS one 2015;10(10):e0140747
Anti-inflammatory Effects of Resveratrol on Hypoxia/Reoxygenation-Induced Alveolar Epithelial Cell Dysfunction.
Liu PL, Chong IW, Lee YC, Tsai JR, Wang HM, Hsieh CC, Kuo HF, Liu WL, Chen YH, Chen HL
Journal of agricultural and food chemistry 2015 Nov 4;63(43):9480-7
Journal of agricultural and food chemistry 2015 Nov 4;63(43):9480-7
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: A549 B: HeLa C: HepG2 D: HCT116 12% SDS PAGE GTX110527 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membranes were blotted with IL6 antibody (GTX110527) diluted at 1:1500 and competitor's antibody (CST#12153) diluted by 1:3000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- IL6 antibody detects IL6 recombinant protein by western blot analysis. Various amounts of protein were separated by 12% SDS-PAGE, and the membranes were blotted with IL6 antibody (GTX110527) diluted at 1:2250 and competitor's antibody (CST#12153) diluted by 1:2000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- IL6 antibody detects IL6 recombinant protein by western blot analysis. Various amounts of protein were separated by 12% SDS-PAGE, and the membranes were blotted with IL6 antibody (GTX110527) diluted at 1:2250 and competitor's antibody (CST#12153) diluted by 1:2000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with IL6 antibody (GTX110527) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of methanol-fixed HeLa, using IL6(GTX110527) antibody at 1:200 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human breast cancer, using IL6(GTX110527) antibody at 1:250 dilution.