AP2316a

antibody from Abcepta

Targeting: GGA1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP2316a

Provider

Abcepta

Proper citation

Abgent Cat#AP2316a, RRID:AB_353114

Product name

GGA1 Antibody (N-term)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

2 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

The anti-GGA1 Pab (Cat. #AP2316a) is used in Western blot to detect GGA1 in mouse kidney tissue lysate (lane 1) and Jurkat cell lysate (lane 2).

Primary Ab dilution

1:1000

Immunocytochemistry

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

"A, N2a cells cotransfected with APP770 –V5 and GGA1 –myc or empty vector were immunostained for APP (Alexa488; green), GGA1 (Cy5; blue), and the Golgi marker GM130 (Cy3; red). B, The same transfection was stained for the endosomal marker EEA1 (Cy3). C, To assess cell-surface localization, APP695 –GFP and GGA1 –myc or empty vector were cotransfected and then immunostained with an Ab to the APP ectodomain on ice without permeabilization (Cy3). Cells were then fixed, permeabilized, and stained for GGA1 (Cy5). (J. Neurosci. 2006 Sep 27;26(39):9913-9922)"

Primary Ab dilution

1:10~50