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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- TA319321 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal anti-GGA-1 antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal anti-GGA-1 antibody
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- GGA1
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µg
- Concentration
- 1.35 mg/mL
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- WB using Anti-GGA1 antibody shows detection of bands at ~100 kDa corresponding to YFP-GGA1 fusion present in a lysate of HEK293 cells overexpressing the recombinant protein (arrowhead). Approximately 35 ug of lysate was separated by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,350. Reaction occurred overnight at 4? followed by washes and reaction with a 1:10,000 dilution of IRDye?800 conjugated Rb-a-Goat IgG [H&L].
- Validation comment
- WB
