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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- NBP2-12728 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal ERK1/2 Antibody
- Antibody type
- Polyclonal
- Description
- Peptide affinity purified. Detects approx 44kda (ERK1) and approx 42kDa (ERK2).
- Reactivity
- Human, Mouse, Rat, Bovine, Chicken/Avian, Drosophila, Sheep, Xenopus
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.025 ml
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ERK1/2 Antibody [NBP2-12728] - analysis of Human Cell line lysates showing detection of ERK1 protein using Rabbit Anti-ERK1 Polyclonal Antibody . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-ERK1 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ERK1/2 Antibody [NBP2-12728] - Tissue: Inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Rabbit Anti-ERK1 Polyclonal Antibody at 1:25000 for 12 hours at 4-C. Secondary Antibody: Biotin Goat Anti-Rabbit at 1:2000 for 1 hour at RT. Counterstain: Methyl Green at 200 uL for 2 min at RT
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ERK1/2 Antibody [NBP2-12728] - Tested on Bouin's fixed paraffin embedded backskin sections of transgenic mice.
