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- Antibody Data
- Antigen structure
- References [38]
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- 11257-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#11257-1-AP, RRID:AB_2139822
- Product name
- ERK1/2 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated ERK1/2 antibody (Cat. #11257-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: FC, IF, IHC, WB,ELISA.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Study on the new anti-atherosclerosis activity of different Herba patriniae through down-regulating lysophosphatidylcholine of the glycerophospholipid metabolism pathway.
Expression of Ubiquitin-specific protease 7 in oral squamous cell carcinoma promotes tumor cell proliferation and invasion.
Uncovering the Pharmacology of Xiaochaihu Decoction in the Treatment of Acute Pancreatitis Based on the Network Pharmacology.
Comparative Efficacy of Haizao Yuhu Decoction Composed of Different Varieties of Glycyrrhiza in Goiter Rats.
Baicalin Alleviates Oxidative Stress and Inflammation in Diabetic Nephropathy via Nrf2 and MAPK Signaling Pathway.
Baicalin ameliorates chronic unpredictable mild stress-induced depression through the BDNF/ERK/CREB signaling pathway.
Neurobehavioral alternations of the female offspring born to polycystic ovary syndrome model rats administered by Chinese herbal medicine.
A network pharmacology approach to reveal the pharmacological targets and biological mechanism of compound kushen injection for treating pancreatic cancer based on WGCNA and in vitro experiment validation.
1,10-Seco-Eudesmane sesquiterpenoids as a new type of anti-neuroinflammatory agents by suppressing TLR4/NF-κB/MAPK pathways.
Berberine-photodynamic therapy sensitizes melanoma cells to cisplatin-induced apoptosis through ROS-mediated P38 MAPK pathways.
Knockdown of RNF6 inhibits HeLa cervical cancer cell growth via suppression of MAPK/ERK signaling.
MiR-1307-5p targeting TRAF3 upregulates the MAPK/NF-κB pathway and promotes lung adenocarcinoma proliferation.
Precise and efficient silencing of mutant KrasG12D by CRISPR-CasRx controls pancreatic cancer progression.
Grape Seed Proanthocyanidins Induce Apoptosis and Cell Cycle Arrest of HepG2 Cells Accompanied by Induction of the MAPK Pathway and NAG-1.
Resveratrol-loaded biopolymer core-shell nanoparticles: bioavailability and anti-inflammatory effects.
Inhibiting PKCβ2 protects HK-2 cells against meglumine diatrizoate and AGEs-induced apoptosis and autophagy.
Sodium tanshinone IIA sulfonate prevents radiation-induced damage in primary rat cardiac fibroblasts.
Hfm1 participates in Golgi-associated spindle assembly and division in mouse oocyte meiosis.
Protection of diabetes-induced kidney injury by phosphocreatine via the regulation of ERK/Nrf2/HO-1 signaling pathway.
Omega-3 polyunsaturated fatty acids alleviate hydrogen sulfide-induced blood-testis barrier disruption in the testes of adult mice.
Vicenin-2 inhibits the Helicobacterium pylori infection associated gastric carcinogenic events through modulation of PI3K/AKT and Nrf2 signaling in GES-1 cells.
MiR-96-5p promotes breast cancer migration by activating MEK/ERK signaling.
PPP5C promotes cell proliferation and survival in human prostate cancer by regulating of the JNK and ERK1/2 phosphorylation.
Lactate enhanced the effect of parathyroid hormone on osteoblast differentiation via GPR81-PKC-Akt signaling.
Soluble epoxide hydrolase inhibition alleviated cognitive impairments via NRG1/ErbB4 signaling after chronic cerebral hypoperfusion induced by bilateral carotid artery stenosis in mice.
Sinomenine hydrochloride attenuates the proliferation, migration, invasiveness, angiogenesis and epithelial-mesenchymal transition of clear-cell renal cell carcinoma cells via targeting Smad in vitro.
Nucleophosmin Regulates Intracellular Oxidative Stress Homeostasis via Antioxidant PRDX6.
Fractalkine/CX3CR1 induces apoptosis resistance and proliferation through the activation of the AKT/NF-κB cascade in pancreatic cancer cells.
HER2-siRNA delivered by EGFR-specific single chain antibody inhibits NSCLC cell proliferation and tumor growth.
SOX9 was involved in TKIs resistance in renal cell carcinoma via Raf/MEK/ERK signaling pathway.
Quantitative proteomic analysis identifies new effectors of FOXM1 involved in breast cancer cell migration.
Fucoidan protects ARPE-19 cells from oxidative stress via normalization of reactive oxygen species generation through the Ca²⁺-dependent ERK signaling pathway.
Polychlorinated biphenyl quinone induces endothelial barrier dysregulation by setting the cross talk between VE-cadherin, focal adhesion, and MAPK signaling.
Tenascin-C induces resistance to apoptosis in pancreatic cancer cell through activation of ERK/NF-κB pathway.
Potential effect of matrix stiffness on the enrichment of tumor initiating cells under three-dimensional culture conditions.
13-acetoxysarcocrassolide induces apoptosis on human gastric carcinoma cells through mitochondria-related apoptotic pathways: p38/JNK activation and PI3K/AKT suppression.
MiR-200c increases the radiosensitivity of non-small-cell lung cancer cell line A549 by targeting VEGF-VEGFR2 pathway.
Radiosensitivity enhancement of human hepatocellular carcinoma cell line SMMC-7721 by sorafenib through the MEK/ERK signal pathway.
Su D, Liao L, Zeng Q, Liao Z, Liu Y, Jin C, Zhu G, Chen C, Yang M, Ai Z, Song Y
Phytomedicine : international journal of phytotherapy and phytopharmacology 2022 Jan;94:153833
Phytomedicine : international journal of phytotherapy and phytopharmacology 2022 Jan;94:153833
Expression of Ubiquitin-specific protease 7 in oral squamous cell carcinoma promotes tumor cell proliferation and invasion.
Yang X, Jin J, Yang J, Zhou L, Mi S, Qi G
Genetics and molecular biology 2021;44(4):e20210058
Genetics and molecular biology 2021;44(4):e20210058
Uncovering the Pharmacology of Xiaochaihu Decoction in the Treatment of Acute Pancreatitis Based on the Network Pharmacology.
Zhan L, Pu J, Hu Y, Xu P, Liang W, Ji C
BioMed research international 2021;2021:6621682
BioMed research international 2021;2021:6621682
Comparative Efficacy of Haizao Yuhu Decoction Composed of Different Varieties of Glycyrrhiza in Goiter Rats.
Chen F, Li N, Xiu L, Liu H, Chen S, He C, Fan A, Yu X, Wang X, Ge C, Huo M, He J, Zhong G
Evidence-based complementary and alternative medicine : eCAM 2021;2021:4343239
Evidence-based complementary and alternative medicine : eCAM 2021;2021:4343239
Baicalin Alleviates Oxidative Stress and Inflammation in Diabetic Nephropathy via Nrf2 and MAPK Signaling Pathway.
Ma L, Wu F, Shao Q, Chen G, Xu L, Lu F
Drug design, development and therapy 2021;15:3207-3221
Drug design, development and therapy 2021;15:3207-3221
Baicalin ameliorates chronic unpredictable mild stress-induced depression through the BDNF/ERK/CREB signaling pathway.
Jia Z, Yang J, Cao Z, Zhao J, Zhang J, Lu Y, Chu L, Zhang S, Chen Y, Pei L
Behavioural brain research 2021 Sep 24;414:113463
Behavioural brain research 2021 Sep 24;414:113463
Neurobehavioral alternations of the female offspring born to polycystic ovary syndrome model rats administered by Chinese herbal medicine.
Zhang X, You L, Zhang X, Wang F, Wang Y, Zhou J, Liu C, Qu F
Chinese medicine 2021 Oct 2;16(1):97
Chinese medicine 2021 Oct 2;16(1):97
A network pharmacology approach to reveal the pharmacological targets and biological mechanism of compound kushen injection for treating pancreatic cancer based on WGCNA and in vitro experiment validation.
Wu C, Huang ZH, Meng ZQ, Fan XT, Lu S, Tan YY, You LM, Huang JQ, Stalin A, Ye PZ, Wu ZS, Zhang JY, Liu XK, Zhou W, Zhang XM, Wu JR
Chinese medicine 2021 Nov 22;16(1):121
Chinese medicine 2021 Nov 22;16(1):121
1,10-Seco-Eudesmane sesquiterpenoids as a new type of anti-neuroinflammatory agents by suppressing TLR4/NF-κB/MAPK pathways.
Tang JJ, Wang MR, Dong S, Huang LF, He QR, Gao JM
European journal of medicinal chemistry 2021 Nov 15;224:113713
European journal of medicinal chemistry 2021 Nov 15;224:113713
Berberine-photodynamic therapy sensitizes melanoma cells to cisplatin-induced apoptosis through ROS-mediated P38 MAPK pathways.
Wang X, Gong Q, Song C, Fang J, Yang Y, Liang X, Huang X, Liu J
Toxicology and applied pharmacology 2021 May 1;418:115484
Toxicology and applied pharmacology 2021 May 1;418:115484
Knockdown of RNF6 inhibits HeLa cervical cancer cell growth via suppression of MAPK/ERK signaling.
Zhu K, Bai H, Mu M, Xue Y, Duan Z
FEBS open bio 2021 Jul;11(7):2041-2049
FEBS open bio 2021 Jul;11(7):2041-2049
MiR-1307-5p targeting TRAF3 upregulates the MAPK/NF-κB pathway and promotes lung adenocarcinoma proliferation.
Du X, Wang S, Liu X, He T, Lin X, Wu S, Wang D, Li J, Huang W, Yang H
Cancer cell international 2020;20:502
Cancer cell international 2020;20:502
Precise and efficient silencing of mutant KrasG12D by CRISPR-CasRx controls pancreatic cancer progression.
Jiang W, Li H, Liu X, Zhang J, Zhang W, Li T, Liu L, Yu X
Theranostics 2020;10(25):11507-11519
Theranostics 2020;10(25):11507-11519
Grape Seed Proanthocyanidins Induce Apoptosis and Cell Cycle Arrest of HepG2 Cells Accompanied by Induction of the MAPK Pathway and NAG-1.
Wang L, Zhan J, Huang W
Antioxidants (Basel, Switzerland) 2020 Nov 28;9(12)
Antioxidants (Basel, Switzerland) 2020 Nov 28;9(12)
Resveratrol-loaded biopolymer core-shell nanoparticles: bioavailability and anti-inflammatory effects.
Liu Y, Liang X, Zou Y, Peng Y, McClements DJ, Hu K
Food & function 2020 May 1;11(5):4014-4025
Food & function 2020 May 1;11(5):4014-4025
Inhibiting PKCβ2 protects HK-2 cells against meglumine diatrizoate and AGEs-induced apoptosis and autophagy.
Jiang W, Zhao W, Ye F, Huang S, Wu Y, Chen H, Zhou R, Fu G
Annals of translational medicine 2020 Mar;8(6):293
Annals of translational medicine 2020 Mar;8(6):293
Sodium tanshinone IIA sulfonate prevents radiation-induced damage in primary rat cardiac fibroblasts.
Zhou D, Yang WK, Zhang YF, Xie JH, Ma L, Yang HD, Li Y, Xie P
Chinese journal of natural medicines 2020 Jun;18(6):436-445
Chinese journal of natural medicines 2020 Jun;18(6):436-445
Hfm1 participates in Golgi-associated spindle assembly and division in mouse oocyte meiosis.
Wang H, Zhong C, Yang R, Yin Y, Tan R, Gao L, Gao C, Cui Y, Pu D, Wu J
Cell death & disease 2020 Jun 30;11(6):490
Cell death & disease 2020 Jun 30;11(6):490
Protection of diabetes-induced kidney injury by phosphocreatine via the regulation of ERK/Nrf2/HO-1 signaling pathway.
Shopit A, Niu M, Wang H, Tang Z, Li X, Tesfaldet T, Ai J, Ahmad N, Al-Azab M, Tang Z
Life sciences 2020 Feb 1;242:117248
Life sciences 2020 Feb 1;242:117248
Omega-3 polyunsaturated fatty acids alleviate hydrogen sulfide-induced blood-testis barrier disruption in the testes of adult mice.
Wang S, Chen Q, Zhang Y, Zheng F, Xue T, Ge X, Ma R, Li X, Wu R, Liang K, Qian Z, Ge Y, Ma J, Yao B
Reproductive toxicology (Elmsford, N.Y.) 2020 Dec;98:233-241
Reproductive toxicology (Elmsford, N.Y.) 2020 Dec;98:233-241
Vicenin-2 inhibits the Helicobacterium pylori infection associated gastric carcinogenic events through modulation of PI3K/AKT and Nrf2 signaling in GES-1 cells.
Zhang Y, Sun J, Dong Y, Shen X, Zhang Z
Journal of biochemical and molecular toxicology 2020 Dec 16;:e22680
Journal of biochemical and molecular toxicology 2020 Dec 16;:e22680
MiR-96-5p promotes breast cancer migration by activating MEK/ERK signaling.
Qin WY, Feng SC, Sun YQ, Jiang GQ
The journal of gene medicine 2020 Aug;22(8):e3188
The journal of gene medicine 2020 Aug;22(8):e3188
PPP5C promotes cell proliferation and survival in human prostate cancer by regulating of the JNK and ERK1/2 phosphorylation.
Lv JM, Chen L, Gao Y, Huang H, Pan XW, Liu X, Chen M, Qu FJ, Li L, Wang JK, Cui XG, Xu DF
OncoTargets and therapy 2018;11:5797-5809
OncoTargets and therapy 2018;11:5797-5809
Lactate enhanced the effect of parathyroid hormone on osteoblast differentiation via GPR81-PKC-Akt signaling.
Wu Y, Wang M, Zhang K, Li Y, Xu M, Tang S, Qu X, Li C
Biochemical and biophysical research communications 2018 Sep 5;503(2):737-743
Biochemical and biophysical research communications 2018 Sep 5;503(2):737-743
Soluble epoxide hydrolase inhibition alleviated cognitive impairments via NRG1/ErbB4 signaling after chronic cerebral hypoperfusion induced by bilateral carotid artery stenosis in mice.
Hao J, Chen Y, Yao E, Liu X
Brain research 2018 Nov 15;1699:89-99
Brain research 2018 Nov 15;1699:89-99
Sinomenine hydrochloride attenuates the proliferation, migration, invasiveness, angiogenesis and epithelial-mesenchymal transition of clear-cell renal cell carcinoma cells via targeting Smad in vitro.
Zhao B, Liu L, Mao J, Liu K, Fan W, Liu J, Zhang Z, Li Q
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2017 Dec;96:1036-1044
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2017 Dec;96:1036-1044
Nucleophosmin Regulates Intracellular Oxidative Stress Homeostasis via Antioxidant PRDX6.
Liu GY, Shi JX, Shi SL, Liu F, Rui G, Li X, Gao LB, Deng XL, Li QF
Journal of cellular biochemistry 2017 Dec;118(12):4697-4707
Journal of cellular biochemistry 2017 Dec;118(12):4697-4707
Fractalkine/CX3CR1 induces apoptosis resistance and proliferation through the activation of the AKT/NF-κB cascade in pancreatic cancer cells.
Wang H, Cai J, Du S, Guo Z, Xin B, Wang J, Wei W, Shen X
Cell biochemistry and function 2017 Aug;35(6):315-326
Cell biochemistry and function 2017 Aug;35(6):315-326
HER2-siRNA delivered by EGFR-specific single chain antibody inhibits NSCLC cell proliferation and tumor growth.
Lu Y, Wang Y, Zhang M, Liu L, Li F, Zhang J, Ye M, Zhao H, Zhao J, Yan B, Yang A, Zhang R, Li X, Ren X
Oncotarget 2016 Apr 26;7(17):23594-607
Oncotarget 2016 Apr 26;7(17):23594-607
SOX9 was involved in TKIs resistance in renal cell carcinoma via Raf/MEK/ERK signaling pathway.
Li XL, Chen XQ, Zhang MN, Chen N, Nie L, Xu M, Gong J, Shen PF, Su ZZ, Weng X, Tan JY, Zhao T, Zeng H, Zhou Q
International journal of clinical and experimental pathology 2015;8(4):3871-81
International journal of clinical and experimental pathology 2015;8(4):3871-81
Quantitative proteomic analysis identifies new effectors of FOXM1 involved in breast cancer cell migration.
Ye X, Zhang Y, He B, Meng Y, Li Y, Gao Y
International journal of clinical and experimental pathology 2015;8(12):15836-44
International journal of clinical and experimental pathology 2015;8(12):15836-44
Fucoidan protects ARPE-19 cells from oxidative stress via normalization of reactive oxygen species generation through the Ca²⁺-dependent ERK signaling pathway.
Li X, Zhao H, Wang Q, Liang H, Jiang X
Molecular medicine reports 2015 May;11(5):3746-52
Molecular medicine reports 2015 May;11(5):3746-52
Polychlorinated biphenyl quinone induces endothelial barrier dysregulation by setting the cross talk between VE-cadherin, focal adhesion, and MAPK signaling.
Zhang P, Feng S, Bai H, Zeng P, Chen F, Wu C, Peng Y, Zhang Q, Zhang Q, Ye Q, Xue Q, Xu X, Song E, Song Y
American journal of physiology. Heart and circulatory physiology 2015 May 15;308(10):H1205-14
American journal of physiology. Heart and circulatory physiology 2015 May 15;308(10):H1205-14
Tenascin-C induces resistance to apoptosis in pancreatic cancer cell through activation of ERK/NF-κB pathway.
Shi M, He X, Wei W, Wang J, Zhang T, Shen X
Apoptosis : an international journal on programmed cell death 2015 Jun;20(6):843-57
Apoptosis : an international journal on programmed cell death 2015 Jun;20(6):843-57
Potential effect of matrix stiffness on the enrichment of tumor initiating cells under three-dimensional culture conditions.
Liu C, Liu Y, Xu XX, Wu H, Xie HG, Chen L, Lu T, Yang L, Guo X, Sun GW, Wang W, Ma XJ, He X
Experimental cell research 2015 Jan 1;330(1):123-34
Experimental cell research 2015 Jan 1;330(1):123-34
13-acetoxysarcocrassolide induces apoptosis on human gastric carcinoma cells through mitochondria-related apoptotic pathways: p38/JNK activation and PI3K/AKT suppression.
Su CC, Chen JY, Din ZH, Su JH, Yang ZY, Chen YJ, Wang RY, Wu YJ
Marine drugs 2014 Oct 23;12(10):5295-315
Marine drugs 2014 Oct 23;12(10):5295-315
MiR-200c increases the radiosensitivity of non-small-cell lung cancer cell line A549 by targeting VEGF-VEGFR2 pathway.
Shi L, Zhang S, Wu H, Zhang L, Dai X, Hu J, Xue J, Liu T, Liang Y, Wu G
PloS one 2013;8(10):e78344
PloS one 2013;8(10):e78344
Radiosensitivity enhancement of human hepatocellular carcinoma cell line SMMC-7721 by sorafenib through the MEK/ERK signal pathway.
Dai XF, Ding J, Zhang RG, Ren JH, Ma CM, Wu G
International journal of radiation biology 2013 Sep;89(9):724-31
International journal of radiation biology 2013 Sep;89(9):724-31
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- A375 cells were subjected to SDS PAGE followed by western blot with 11257-1-AP(ERK1 antibody) at dilution of 1:2000
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human colon cancer using 11257-1-AP(ERK1 antibody) at dilution of 1:50 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The ERK1/2 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human ERK1/2. This antibody recognizes human, mouse antigen. The ERK1/2 antibody has been validated for the following applications: ELISA, WB, IHC analysis.
