Antibody data
- Antibody Data
- Antigen structure
- References [16]
- Comments [0]
- Validations
- Immunocytochemistry [1]
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- Product number
- 4370 - Provider product page
- Provider
- Cell Signaling Technology, Inc
- Proper citation
- Cell Signaling Technology Cat#4370, RRID:AB_2315112
- Product name
- Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Drosophila, Hamster, Porcine, Simian, Yeast, Other
- Host
- Rabbit
- Storage
- -20°C
Submitted references Stage-specific roles for CXCR4 signaling in murine hematopoietic stem/progenitor cells in the process of bone marrow repopulation.
c-Jun N-terminal kinase phosphorylation of heterogeneous nuclear ribonucleoprotein K regulates vertebrate axon outgrowth via a posttranscriptional mechanism.
BIM expression in treatment-naive cancers predicts responsiveness to kinase inhibitors.
PAR1- and PAR2-induced innate immune markers are negatively regulated by PI3K/Akt signaling pathway in oral keratinocytes.
Renal tumour suppressor function of the Birt-Hogg-Dubé syndrome gene product folliculin.
Met activation in non-small cell lung cancer is associated with de novo resistance to EGFR inhibitors and the development of brain metastasis.
Ligation of beta4 integrins activates PKB/Akt and ERK1/2 by distinct pathways-relevance of the keratin filament.
Akt-RSK-S6 kinase signaling networks activated by oncogenic receptor tyrosine kinases.
Interleukin-32 promotes osteoclast differentiation but not osteoclast activation.
Nf1 haploinsufficiency and Icsbp deficiency synergize in the development of leukemias.
Augmented TLR9-induced Btk activation in PIR-B-deficient B-1 cells provokes excessive autoantibody production and autoimmunity.
Genetic evidence for the role of Erk activation in a lymphoproliferative disease of mice.
Kinome profiling of chondrosarcoma reveals SRC-pathway activity and dasatinib as option for treatment.
Therapeutic targeting of neuropilin-2 on colorectal carcinoma cells implanted in the murine liver.
Effective use of PI3K and MEK inhibitors to treat mutant Kras G12D and PIK3CA H1047R murine lung cancers.
The Rac activator Tiam1 prevents keratinocyte apoptosis by controlling ROS-mediated ERK phosphorylation.
Lai CY, Yamazaki S, Okabe M, Suzuki S, Maeyama Y, Iimura Y, Onodera M, Kakuta S, Iwakura Y, Nojima M, Otsu M, Nakauchi H
Stem cells (Dayton, Ohio) 2014 Jul;32(7):1929-42
Stem cells (Dayton, Ohio) 2014 Jul;32(7):1929-42
c-Jun N-terminal kinase phosphorylation of heterogeneous nuclear ribonucleoprotein K regulates vertebrate axon outgrowth via a posttranscriptional mechanism.
Hutchins EJ, Szaro BG
The Journal of neuroscience : the official journal of the Society for Neuroscience 2013 Sep 11;33(37):14666-80
The Journal of neuroscience : the official journal of the Society for Neuroscience 2013 Sep 11;33(37):14666-80
BIM expression in treatment-naive cancers predicts responsiveness to kinase inhibitors.
Faber AC, Corcoran RB, Ebi H, Sequist LV, Waltman BA, Chung E, Incio J, Digumarthy SR, Pollack SF, Song Y, Muzikansky A, Lifshits E, Roberge S, Coffman EJ, Benes CH, Gómez HL, Baselga J, Arteaga CL, Rivera MN, Dias-Santagata D, Jain RK, Engelman JA
Cancer discovery 2011 Sep;1(4):352-65
Cancer discovery 2011 Sep;1(4):352-65
PAR1- and PAR2-induced innate immune markers are negatively regulated by PI3K/Akt signaling pathway in oral keratinocytes.
Rohani MG, DiJulio DH, An JY, Hacker BM, Dale BA, Chung WO
BMC immunology 2010 Oct 28;11:53
BMC immunology 2010 Oct 28;11:53
Renal tumour suppressor function of the Birt-Hogg-Dubé syndrome gene product folliculin.
Hudon V, Sabourin S, Dydensborg AB, Kottis V, Ghazi A, Paquet M, Crosby K, Pomerleau V, Uetani N, Pause A
Journal of medical genetics 2010 Mar;47(3):182-9
Journal of medical genetics 2010 Mar;47(3):182-9
Met activation in non-small cell lung cancer is associated with de novo resistance to EGFR inhibitors and the development of brain metastasis.
Benedettini E, Sholl LM, Peyton M, Reilly J, Ware C, Davis L, Vena N, Bailey D, Yeap BY, Fiorentino M, Ligon AH, Pan BS, Richon V, Minna JD, Gazdar AF, Draetta G, Bosari S, Chirieac LR, Lutterbach B, Loda M
The American journal of pathology 2010 Jul;177(1):415-23
The American journal of pathology 2010 Jul;177(1):415-23
Ligation of beta4 integrins activates PKB/Akt and ERK1/2 by distinct pathways-relevance of the keratin filament.
Kippenberger S, Hofmann M, Zöller N, Thaçi D, Müller J, Kaufmann R, Bernd A
Biochimica et biophysica acta 2010 Aug;1803(8):940-50
Biochimica et biophysica acta 2010 Aug;1803(8):940-50
Akt-RSK-S6 kinase signaling networks activated by oncogenic receptor tyrosine kinases.
Moritz A, Li Y, Guo A, Villén J, Wang Y, MacNeill J, Kornhauser J, Sprott K, Zhou J, Possemato A, Ren JM, Hornbeck P, Cantley LC, Gygi SP, Rush J, Comb MJ
Science signaling 2010 Aug 24;3(136):ra64
Science signaling 2010 Aug 24;3(136):ra64
Interleukin-32 promotes osteoclast differentiation but not osteoclast activation.
Mabilleau G, Sabokbar A
PloS one 2009;4(1):e4173
PloS one 2009;4(1):e4173
Nf1 haploinsufficiency and Icsbp deficiency synergize in the development of leukemias.
Koenigsmann J, Rudolph C, Sander S, Kershaw O, Gruber AD, Bullinger L, Schlegelberger B, Carstanjen D
Blood 2009 May 7;113(19):4690-701
Blood 2009 May 7;113(19):4690-701
Augmented TLR9-induced Btk activation in PIR-B-deficient B-1 cells provokes excessive autoantibody production and autoimmunity.
Kubo T, Uchida Y, Watanabe Y, Abe M, Nakamura A, Ono M, Akira S, Takai T
The Journal of experimental medicine 2009 Aug 31;206(9):1971-82
The Journal of experimental medicine 2009 Aug 31;206(9):1971-82
Genetic evidence for the role of Erk activation in a lymphoproliferative disease of mice.
Miyaji M, Kortum RL, Surana R, Li W, Woolard KD, Simpson RM, Samelson LE, Sommers CL
Proceedings of the National Academy of Sciences of the United States of America 2009 Aug 25;106(34):14502-7
Proceedings of the National Academy of Sciences of the United States of America 2009 Aug 25;106(34):14502-7
Kinome profiling of chondrosarcoma reveals SRC-pathway activity and dasatinib as option for treatment.
Schrage YM, Briaire-de Bruijn IH, de Miranda NF, van Oosterwijk J, Taminiau AH, van Wezel T, Hogendoorn PC, Bovée JV
Cancer research 2009 Aug 1;69(15):6216-22
Cancer research 2009 Aug 1;69(15):6216-22
Therapeutic targeting of neuropilin-2 on colorectal carcinoma cells implanted in the murine liver.
Gray MJ, Van Buren G, Dallas NA, Xia L, Wang X, Yang AD, Somcio RJ, Lin YG, Lim S, Fan F, Mangala LS, Arumugam T, Logsdon CD, Lopez-Berestein G, Sood AK, Ellis LM
Journal of the National Cancer Institute 2008 Jan 16;100(2):109-20
Journal of the National Cancer Institute 2008 Jan 16;100(2):109-20
Effective use of PI3K and MEK inhibitors to treat mutant Kras G12D and PIK3CA H1047R murine lung cancers.
Engelman JA, Chen L, Tan X, Crosby K, Guimaraes AR, Upadhyay R, Maira M, McNamara K, Perera SA, Song Y, Chirieac LR, Kaur R, Lightbown A, Simendinger J, Li T, Padera RF, García-Echeverría C, Weissleder R, Mahmood U, Cantley LC, Wong KK
Nature medicine 2008 Dec;14(12):1351-6
Nature medicine 2008 Dec;14(12):1351-6
The Rac activator Tiam1 prevents keratinocyte apoptosis by controlling ROS-mediated ERK phosphorylation.
Rygiel TP, Mertens AE, Strumane K, van der Kammen R, Collard JG
Journal of cell science 2008 Apr 15;121(Pt 8):1183-92
Journal of cell science 2008 Apr 15;121(Pt 8):1183-92
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Supportive validation
- Submitted by
- tatarskii@gmail.com
- Main image
- Experimental details
- C2C12, seeded in DMEM with 10% FBS, transferred to serum-free medium for 16 hours. Added 100 ng/ml EGF for 10 minutes, then fixed.
- Sample type
- C2C12, mouse myoblast cell line
- Primary Ab dilution
- 1:200
- Conjugate
- Unconjugated
- Secondary Ab
- http://www.cellsignal.com/products/4412.html
- Secondary Ab dilution
- 1:1000
- Protocol
- Protocol