- 13-8600 - Invitrogen Antibodies MAPK3 antibody

Submitted references Curcumin Enhances Radiosensitization of Nasopharyngeal Carcinoma via Mediating Regulation of Tumor Stem-like Cells by a CircRNA Network.

Zhu D, Shao M, Yang J, Fang M, Liu S, Lou D, Gao R, Liu Y, Li A, Lv Y, Mo Z, Fan Q
Journal of Cancer 2020;11(8):2360-2370

Pulsed focused ultrasound enhances the therapeutic effect of mesenchymal stromal cell-derived extracellular vesicles in acute kidney injury.

Ullah M, Liu DD, Rai S, Razavi M, Concepcion W, Thakor AS
Stem cell research & therapy 2020 Sep 14;11(1):398

Extracts of Physalis peruviana Protect Astrocytic Cells Under Oxidative Stress With Rotenone.

Areiza-Mazo N, Robles J, Zamudio-Rodriguez JA, Giraldez L, Echeverria V, Barrera-Bailon B, Aliev G, Sahebkar A, Ashraf GM, Barreto GE
Frontiers in chemistry 2018;6:276

ERK2 mediates inner hair cell survival and decreases susceptibility to noise-induced hearing loss.

Kurioka T, Matsunobu T, Satoh Y, Niwa K, Endo S, Fujioka M, Shiotani A
Scientific reports 2015 Nov 18;5:16839

Loss of NGF-TrkA signaling from the CNS is not sufficient to induce cognitive impairments in young adult or intermediate-aged mice.

Müller M, Triaca V, Besusso D, Costanzi M, Horn JM, Koudelka J, Geibel M, Cestari V, Minichiello L
The Journal of neuroscience : the official journal of the Society for Neuroscience 2012 Oct 24;32(43):14885-98

TrkB modulates fear learning and amygdalar synaptic plasticity by specific docking sites.

Musumeci G, Sciarretta C, Rodríguez-Moreno A, Al Banchaabouchi M, Negrete-Díaz V, Costanzi M, Berno V, Egorov AV, von Bohlen Und Halbach O, Cestari V, Delgado-García JM, Minichiello L
The Journal of neuroscience : the official journal of the Society for Neuroscience 2009 Aug 12;29(32):10131-43

PAC1 is a direct transcription target of E2F-1 in apoptotic signaling.

Wu J, Jin YJ, Calaf GM, Huang WL, Yin Y
Oncogene 2007 Oct 4;26(45):6526-35

Roscovitine targets, protein kinases and pyridoxal kinase.

Bach S, Knockaert M, Reinhardt J, Lozach O, Schmitt S, Baratte B, Koken M, Coburn SP, Tang L, Jiang T, Liang DC, Galons H, Dierick JF, Pinna LA, Meggio F, Totzke F, Schächtele C, Lerman AS, Carnero A, Wan Y, Gray N, Meijer L
The Journal of biological chemistry 2005 Sep 2;280(35):31208-19

Distinct mechanisms mediate the initial and sustained phases of cell migration in epidermal growth factor receptor-overexpressing cells.

Kruger JS, Reddy KB
Molecular cancer research : MCR 2003 Sep;1(11):801-9

Adipocyte functions are modulated by cell size change: potential involvement of an integrin/ERK signalling pathway.

Farnier C, Krief S, Blache M, Diot-Dupuy F, Mory G, Ferre P, Bazin R
International journal of obesity and related metabolic disorders : journal of the International Association for the Study of Obesity 2003 Oct;27(10):1178-86

Regulation of SPIN90 phosphorylation and interaction with Nck by ERK and cell adhesion.

Lim CS, Kim SH, Jung JG, Kim JK, Song WK
The Journal of biological chemistry 2003 Dec 26;278(52):52116-23

Alpha 7 nicotinic acetylcholine receptors mediate beta-amyloid peptide-induced tau protein phosphorylation.

Wang HY, Li W, Benedetti NJ, Lee DH
The Journal of biological chemistry 2003 Aug 22;278(34):31547-53

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on whole cell extracts (30 µg lysate) of RSC96 (Lane 1), HeLa (Lane 2), H1975 (Lane 3), HCT116 (Lane 4), HT-29 (Lane 5), PC12 (Lane 6), MCF7 (Lane 7) and NIH/3T3 (Lane 8). The blot was probed with Anti-ERK1 Mouse Monoclonal Antibody (Product # 13-8600, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 44 kDa band corresponding to ERK1 was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane using the wet transfer system. The membrane was probed with the relevant primary and secondary antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of ERK1 was performed using 70% confluent A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ERK1 (ERK-6B11) Mouse Monoclonal Antibody (Product # 13-8600) at 1:250 dilution in 0.1% BSA and incubated overnight at 4 degree Celsius and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry analysis of ERK1 was done on HeLa cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were labeled with ERK1 Mouse Monoclonal Antibody (138600, red histogram) or with mouse isotype control (pink histogram) at 2 µg - 4 µg/million cells in 2.5% BSA. After incubation at room temperature for 2 - 3 hours, the cells were labeled with Alexa Fluor® 488 Rabbit Anti-Mouse Secondary Antibody (A11059) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Attune® Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 1 The phosphorylation levels of ERK1 and ERK2 are increased after noise exposure. ( a ) Representative western blots for the phosphorylation levels of ERK1 and ERK2 in the modiolus (MO), lateral wall (LW), and neurosensory epithelium (NSE) 5 h after noise exposure. ( b ) The phosphorylation levels of ERK1 and ERK2 ( b ) in the cochlea were upregulated 5 h after noise exposure. Specifically, phosphorylation levels of ERK1 and ERK2 in the LW and NSE were significantly upregulated 5 h after noise exposure (n = 4 for each group). beta-actin served as the control for protein loading. Error bars show SE. (Mann-Whitney U-test, *p < 0.05, **p < 0.01). ( c ) In naive mice, p-ERK1/2 was not observed in the organ of Corti. By contrast, at 5 h after noise exposure, p-ERK1/2 was detected in IHCs, OHCs, and SCs, including IPCs and DCs. IHC,; inner hair cell; OHC, outer hair cell; SC, supporting cell; IPC, inner pillar cell; DC, Deiter's cell. Scale bar, 20 mum. ( d ) In naive mice, p-ERK1/2 was not observed in the MO and LW. By contrast, p-ERK1/2 was detected in spiral ganglion neurons (SGNs) and spiral ligaments of the LW. Scale bar, 50 mum.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 6 Signaling pathway analysis. a Western blot and protein quantification showing the expression of p-ERK, ERK, p-MEK, MEK, and beta-actin in kidney tissue. b Western blot and protein quantification showing the expression of p-Akt, Akt, and beta-actin in kidney tissue. c Western blot and protein quantification showing the expression of SIRT3, eNOS, and beta-actin in kidney tissue. Each group has n = 3 mice. Significant difference a p < 0.05: relative to untreated control; b p < 0.05: relative to AKI; c p < 0.05: relative to AKI-EV; d p < 0.05: relative to AKI-EV-pFUS

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 3 ERK2 expression was abrogated in the HC of HC-E2CKO mice. ( a ) Whole mount preparations and cross sections of cochleae from HC-E2CKO and control mice. IHCs, OHCs, IPCs, and DCs showed ERK2 immunoreactivity in control cochleae while HC-E2CKO cochleae exhibited no staining in HCs and weak staining in SCs. ERK1 expression was observed in OHCs and SCs and was unchanged in both HC-E2CKO and control mice. Scale bar, 20 mum. ( b ) Representative western blots of the NSE from control and HC-E2CKO mice. The 44-kDa (Fig. 3b, left) and 42-kDa (Fig. 3b, middle) bands were detected using anti-ERK1 and anti-ERK2 antibodies, respectively. The 42-kDa band from HC-E2CKO mice was reduced compared to that of controls (Fig. 3b, right). ( c , d ) No prominent difference in ERK1 and ERK2 expression was observed in the lateral wall ( c ) and spiral ganglion ( d ) between control and HC-E2CKO mice. Scale bar, 50 mum. ( e , f ) Whole mount preparations were stained with myosin7a (green) and CtBP2 (red) (e). Scale bar, 20 mum. No prominent difference in the number of synaptic ribbons was observed at any frequency (n = 4 for each group) ( f ). IHC, inner hair cell; OHC, outer hair cell; IPC, inner pillar cell; DC, Deiter's cell, SC, supporting cell; Myo7a, myosin7a. Data represent mean values; error bars show SE.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 4 p-ERK1/2 was detected in OHCs and SCs but not in IHCs after noise exposure in HC-E2CKO mice. In naive HC-E2CKO mice, p-ERK1/2 was not detected in the organ of Corti. By contrast, at 5 h after noise exposure in HC-E2CKO mice, the immunoreactive signal of p-ERK1/2 was detected in OHCs and DCs, but not in IHCs. IHC; inner hair cell. OHC, outer hair cell; DC, Deiter's cell. Scale bar, 20 mum.

13-8600

Antibody data