F48590

antibody from NSJ Bioreagents

Targeting: MAPK3 ERK1, p44erk1, p44mapk, PRKM3

Western blot (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

F48590

Provider

NSJ Bioreagents

Product name

Phospho-ERK1/2 Antibody [p-ERK1/2] (T202/Y204)

Antibody type

Polyclonal

Antigen

This phospho-ERK1/2 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pT202/pY204 of human ERK1/2.

Description

Antigen affinity purified antibody

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Vial size

80 µl, 400 µl

Storage

Aliquot the phospho-ERK1/2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot analysis of phospho-ERK1/2 antibody and HAOSMC cell lysate noninduced (lane 1) or induced with the PDGF (lane 2).

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot analysis of phospho-ERK1/2 antibody and HBEpC cell lysate, noninduced (lane 1) or induced with the EGF (lane 2).

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human HeLa and mouse NIH3T3 cells treated with TPA (200nM) and PDGF (100ng/ml) using phospho-ERK1/2 antibody.

Other assay

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Dot blot analysis of phospho-ERK1/2 antibody. 50ng of Bi-phos-peptide or nonphosrylated peptide per dot were spotted.