F48590

antibody from NSJ Bioreagents

Targeting: MAPK3 ERK1, p44erk1, p44mapk, PRKM3

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

F48590

Provider

NSJ Bioreagents

Product name

Phospho-ERK1/2 Antibody [p-ERK1/2] (T202/Y204)

Antibody type

Polyclonal

Description

This highly specific Phospho-ERK1/2 antibody is suitable for use in Dot blot/Western blot/ELISA applications with human and mouse samples.

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Unconjugated

Vial size

0.08 ml, 0.4 ml

Concentration

In 1X PBS, pH 7.4, with 0.09% sodium azide

Storage

Aliquot the phospho-ERK1/2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot analysis of phospho-ERK1/2 antibody and HAOSMC cell lysate noninduced (lane 1) or induced with the PDGF (lane 2).

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot analysis of phospho-ERK1/2 antibody and HBEpC cell lysate, noninduced (lane 1) or induced with the EGF (lane 2).

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human HeLa and mouse NIH3T3 cells treated with TPA (200nM) and PDGF (100ng/ml) using phospho-ERK1/2 antibody.

Other assay

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Dot blot analysis of phospho-ERK1/2 antibody. 50ng of Bi-phos-peptide or nonphosrylated peptide per dot were spotted.