686903

antibody from BioLegend

Targeting: MAPK3 ERK1, p44erk1, p44mapk, PRKM3

Immunocytochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

686903

Provider

BioLegend

Proper citation

BioLegend Cat#686903, RRID:AB_2617023

Product name

Alexa Fluor® 594 anti-ERK1/2

Antibody type

Monoclonal

Antigen

Partial human ERK1 recombinant protein (180-379 a.a.) expressed in E. coli.

Reactivity

Human, Mouse

Host

Rat

Conjugate

Yellow dye

Antibody clone number

W15133B

Vial size

25 µg

Concentration

0.5 μ/ml

Storage

2°C-8°C

Handling

Ambient RT

Other assay

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

HeLa cells were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for three minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with 2.5 µg/ml Alexa Fluor® 594 anti-ERK1/2 antibody (clone W15133B) overnight at 4°C. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.

Conjugate

Yellow dye