M00104

antibody from Boster Biological Technology

Targeting: MAPK3 ERK1, p44erk1, p44mapk, PRKM3

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

M00104

Provider

Boster Biological Technology

Product name

Anti-ERK1 MAPK3 Rabbit Monoclonal Antibody

Antibody type

Monoclonal

Description

Monoclonal antibody for ERK1/MAPK3 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: Flow Cytometry, IP, IF, IHC, ICC, WB. Reactive species: Human, Mouse ERK1/MAPK3 information: Molecular Weight: 43136 MW; Subcellular Localization: Cytoplasm. Nucleus. Autophosphorylation at Thr-207 promotes nuclear localization.

Reactivity

Human, Mouse

Host

Rabbit

Antibody clone number

ADF-13

Vial size

100ug/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. Avoid repeated freezing and thawing.

References

Submitted references

Transcription factor Runx2 changes the expression of some matricellular proteins in metastatic breast cancer cells.

Binay S, Kaptan E
Molecular biology reports 2022 Jul;49(7):6433-6441

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using ERK1 Antibody(M00104)MAPK3 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MAPK3 Antibody (M00104)overnight at 4?? Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37?? The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image