M00104-1

antibody from Boster Biological Technology

Targeting: MAPK3 ERK1, p44erk1, p44mapk, PRKM3

Provider product page for M00104-1

Western blot

Immunocytochemistry

Immunoprecipitation

Flow cytometry

Antibody data

Antibody Data
Antigen structure
References [35]
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Validations
Western blot [1]

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Product number: M00104-1 - Provider product page
Provider: Boster Biological Technology
Product name: Anti-ERK1/2 MAPK3 Rabbit Monoclonal Antibody
Antibody type: Monoclonal
Description: Monoclonal antibody for ERK1/MAPK3 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: Flow Cytometry, IP, IF, ICC, WB. Reactive species: Human, Mouse, Rat ERK1/MAPK3 information: Molecular Weight: 43136 MW; Subcellular Localization: Cytoplasm. Nucleus. Autophosphorylation at Thr-207 promotes nuclear localization.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Antibody clone number: DFF-13
Vial size: 100ug/vial
Concentration: 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
Storage: At -20°C for one year. Avoid repeated freezing and thawing.

MAPK3 protein structure - M00104-1 shown in red.

Submitted references Involvement of PI3K/AKT Pathway in the Rapid Antidepressant Effects of Crocetin in Mice with Depression-Like Phenotypes.

Lin S, Chen Z, Wu Z, Fei F, Xu Z, Tong Y, Sun W, Wang P
Neurochemical research 2024 Feb;49(2):477-491

The mechanism of blood coagulation induced by sodium dehydroacetate via the regulation of the mTOR/ERK pathway in rats.

Zhang M, Zhang Q, Zhao W, Chen X, Zhang Y
Toxicology letters 2024 Feb;392:1-11

Rosavin Alleviates LPS-Induced Acute Lung Injure by Modulating the TLR-4/NF-κB/MAPK Singnaling Pathways.

Liu QH, Zhang K, Feng SS, Zhang LJ, Li SY, Wang HY, Wang JH
International journal of molecular sciences 2024 Feb 3;25(3)

Active fractions from Jingfang Baidu Powder alleviate Klebsiella-induced Pneumonia by inhibiting TLR4/Myd88-ERK signaling pathway.

Sun C, Xu Y, Xu G, Ji X, Jiang P, He Y
Journal of ethnopharmacology 2024 Aug 10;330:118067

Dopamine receptor 1 on CaMKII-positive neurons within claustrum mediates adolescent cocaine exposure-induced anxiety-like behaviors and electro-acupuncture therapy.

Chen L, Liu Z, Zhao Z, Du D, Pan W, Wei X, Nie J, Ge F, Ding J, Fan Y, Kim HY, Guan X
Theranostics 2023;13(10):3149-3164

mPFC DUSP1 mediates adolescent cocaine exposure-induced higher sensitivity to drug in adulthood.

Wei X, Chang J, Cheng Z, Chen W, Guo H, Liu Z, Mai Y, Hu T, Zhang Y, Cai Q, Ge F, Fan Y, Guan X
EMBO reports 2023 Sep 6;24(9):e56981

Paternal methamphetamine exposure induces higher sensitivity to methamphetamine in male offspring through driving ADRB1 on CaMKII-positive neurons in mPFC.

Zheng Y, Liu D, Guo H, Chen W, Liu Z, Li Z, Hu T, Zhang Y, Li X, Zhao Z, Cai Q, Ge F, Fan Y, Guan X
Translational psychiatry 2023 Oct 19;13(1):324

Patchouli alcohol against renal fibrosis of spontaneously hypertensive rats via Ras/Raf-1/ERK1/2 signalling pathway.

Li J, Hu G, Liu W, Cao X, Chen G, Peng F, Xiaofang X, Peng C
The Journal of pharmacy and pharmacology 2023 Jul 5;75(7):995-1010

RNF113A targeted by miR-197 promotes proliferation and inhibits autophagy via CXCR4/CXCL12/AKT/ERK/Beclin1 axis in cervical cancer.

Zhang Q, Song J, Cao L, Sun M, Xu T, Yang S, Li S, Wang H, Fu X
Experimental cell research 2023 Jul 1;428(1):113632

Accelerated osteogenesis of bone graft by optimizing the bone microenvironment formed by electrical signals dependent on driving micro vibration stimulation.

Wu Y, Wu J, Huang X, Zhu X, Zhi W, Wang J, Sun D, Chen X, Zhu X, Zhang X
Materials today. Bio 2023 Dec;23:100891

Identification of several inflammation-related genes based on bioinformatics and experiments.

Wang S, Zhang Z, Liang J, Li K, Bo L, Zhan H, Hong X, Hu J, Yang Qian L, Liu X, Zhang B
International immunopharmacology 2023 Aug;121:110409

Ameliorative effects of epigallocatechin-3-gallate nanoparticles on 2,4-dinitrochlorobenzene induced atopic dermatitis: A potential mechanism of inflammation-related necroptosis.

Han M, Wang X, Wang J, Lang D, Xia X, Jia Y, Chen Y
Frontiers in nutrition 2022;9:953646

Nuciferine Inhibits TMEM16A in Dietary Adjuvant Therapy for Lung Cancer.

Bai X, Liu X, Li S, An H, Kang X, Guo S
Journal of agricultural and food chemistry 2022 Mar 30;70(12):3687-3696

Fumonisin B(1) induces chicken heterophil extracellular traps mediated by PAD4 enzyme and P2 × 1 receptor.

Wu Z, Zhu X, Li P, Wang X, Sun Y, Fu Y, Wang J, Yang Z, Zhou E
Poultry science 2022 Jan;101(1):101550

Rhodiola rosea L. Attenuates Cigarette Smoke and Lipopolysaccharide-Induced COPD in Rats via Inflammation Inhibition and Antioxidant and Antifibrosis Pathways.

Cui H, Liu X, Zhang J, Zhang K, Yao D, Dong S, Feng S, Yang L, Li Y, Wang H, Huang J, Wang J
Evidence-based complementary and alternative medicine : eCAM 2021;2021:6103158

Effects of combination treatment with transcranial magnetic stimulation and bone marrow mesenchymal stem cell transplantation or Raf inhibition on spinal cord injury in rats.

Feng S, Wang S, Sun S, Su H, Zhang L
Molecular medicine reports 2021 Apr;23(4)

Iridoid glycosides from Morinda officinalis How. exert anti-inflammatory and anti-arthritic effects through inactivating MAPK and NF-κB signaling pathways.

Zhang Q, Zhang JH, He YQ, Zhang QL, Zhu B, Shen Y, Liu MQ, Zhu LL, Xin HL, Qin LP, Zhang QY
BMC complementary medicine and therapies 2020 Jun 5;20(1):172

Glucagon-like peptide 1 treatment reverses vascular remodelling by downregulating matrix metalloproteinase 1 expression through inhibition of the ERK1/2/NF-κB signalling pathway.

Fan SH, Xiong QF, Wang L, Zhang LH, Shi YW
Molecular and cellular endocrinology 2020 Dec 1;518:111005

Tetrandrine attenuates hyperoxia-induced lung injury in newborn rats via NF-κB p65 and ERK1/2 pathway inhibition.

Jiao B, Tang Y, Liu S, Guo C
Annals of translational medicine 2020 Aug;8(16):1018

Endosulfan promotes cell migration via PTP4A3-mediated signaling pathways in HUVECs.

Li H, Liu S, Hu Y, Zhao B, Sun Y, Xu D
Ecotoxicology and environmental safety 2020 Apr 1;192:110267

Medical findings of nasopharyngeal carcinoma patients and anti-tumor benefits of formononetin.

Ying K, Liu Y, Zhang C, Shangguan M
European journal of pharmacology 2019 Oct 15;861:172619

Reciprocal stimulating effects of bFGF and FSH on chicken primordial follicle activation through AKT and ERK pathway.

Guo C, Zhang G, Lin X, Zhao D, Zhang C, Mi Y
Theriogenology 2019 Jul 1;132:27-35

Polyamine analogue QMA attenuated ischemic injury in MCAO rats via ERK and Akt activated Nrf2/HO-1 signaling pathway.

Cen J, Zhao N, Huang WW, Liu L, Xie YY, Gan Y, Wang CJ, Ji BS
European journal of pharmacology 2019 Feb 5;844:165-174

Association of astragaloside IV-inhibited autophagy and mineralization in vascular smooth muscle cells with lncRNA H19 and DUSP5-mediated ERK signaling.

Song Z, Wei D, Chen Y, Chen L, Bian Y, Shen Y, Chen J, Pan Y
Toxicology and applied pharmacology 2019 Feb 1;364:45-54

Grape Seed Procyanidins Attenuates Cisplatin-induced Human Embryonic Renal Cell Cytotoxicity by Modulating Heme Oxygenase-1 in Vitro.

Han H, Wang H, Du Y, Gao L
Cell biochemistry and biophysics 2019 Dec;77(4):367-377

Blockage of High-Affinity Choline Transporter Increases Visceral Hypersensitivity in Rats with Chronic Stress.

Zhao C, Lin M, Pan Y, Yu B
Gastroenterology research and practice 2018;2018:9252984

TMEM88 mediates inflammatory cytokines secretion by regulating JNK/P38 and canonical Wnt/β-catenin signaling pathway in LX-2 cells.

Xu T, Pan LX, Ge YX, Li P, Meng XM, Huang C, Li J
Inflammopharmacology 2018 Oct;26(5):1339-1348

A cardiac glycoside HTF-1 isolated from Helleborus thibetanus Franch displays potent in vitro anti-cancer activity via caspase-9, MAPK and PI3K-Akt-mTOR pathways.

Ma L, Meng Y, Tu C, Cao X, Wang H, Li Y, Man S, Zhou J, Li M, Liu Z, Su Y
European journal of medicinal chemistry 2018 Oct 5;158:743-752

Protection of insulin‑like growth factor 1 on experimental peripheral neuropathy in diabetic mice.

Wang H, Zhang H, Cao F, Lu J, Tang J, Li H, Zhang Y, Feng B, Tang Z
Molecular medicine reports 2018 Nov;18(5):4577-4586

CXCL13 inhibition induce the apoptosis of MDA-MB-231 breast cancer cells through blocking CXCR5/ERK signaling pathway.

Ma JJ, Jiang L, Tong DY, Ren YN, Sheng MF, Liu HC
European review for medical and pharmacological sciences 2018 Dec;22(24):8755-8762

Buyang Huanwu Decoction Ameliorates Poststroke Depression via Promoting Neurotrophic Pathway Mediated Neuroprotection and Neurogenesis.

Luo L, Deng S, Yi J, Zhou S, She Y, Liu B
Evidence-based complementary and alternative medicine : eCAM 2017;2017:4072658

Autocrine and paracrine STIP1 signaling promote osteolytic bone metastasis in renal cell carcinoma.

Wang J, You H, Qi J, Yang C, Ren Y, Cheng H
Oncotarget 2017 Mar 7;8(10):17012-17026

Afatinib inhibits proliferation and invasion and promotes apoptosis of the T24 bladder cancer cell line.

Tang Y, Zhang X, Qi F, Chen M, Li Y, Liu L, He W, Li Z, Zu X
Experimental and therapeutic medicine 2015 May;9(5):1851-1856

Cucurbitacin B inhibits growth and induces apoptosis through the JAK2/STAT3 and MAPK pathways in SH‑SY5Y human neuroblastoma cells.

Zheng Q, Liu Y, Liu W, Ma F, Zhou Y, Chen M, Chang J, Wang Y, Yang G, He G
Molecular medicine reports 2014 Jul;10(1):89-94

Panax notoginseng saponins promote osteogenic differentiation of bone marrow stromal cells through the ERK and P38 MAPK signaling pathways.

Li XD, Liu ZY, Chang B, Liu DX, Chen B, Guo C, Wang YG, Xu JK, Huang DY, Du SX
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2011;28(2):367-76

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Submitted by: Boster Biological Technology (provider)
Main image
Experimental details: Western blot analysis of ERK1/2 Antibody expression in HepG2 whole cell lysates (M00104-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAPK3 monoclonal antibody (Catalog # M00104-1) overnight at 4
Additional image