ABIN359630

antibody from antibodies-online

Targeting: MAPK3 ERK1, p44erk1, p44mapk, PRKM3

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

ABIN359630

Provider

antibodies-online

Product name

anti-Mitogen-Activated Protein Kinase 3 (MAPK3) (N-Term) antibody

Antibody type

Polyclonal

Antigen

This antibody is generated from rabbits immunized with a KLH conjugated syntheticpeptide selected from the N-terminal region of human ERK1.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Vial size

0.1 mg

Concentration

0.25 mg/ml

Storage

Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. Caution: This product is for research use only. Not for use in diagnostic or therapeutic procedures.

References

Submitted references

Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.

Strausberg RL, Feingold EA, Grouse LH, Derge JG, Klausner RD, Collins FS, Wagner L, Shenmen CM, Schuler GD, Altschul SF, Zeeberg B, Buetow KH, Schaefer CF, Bhat NK, Hopkins RF, Jordan H, Moore T, Max SI, Wang J, Hsieh F, Diatchenko L, Marusina K, Farmer AA, Rubin GM, Hong L, Stapleton M, Soares MB, Bonaldo MF, Casavant TL, Scheetz TE, Brownstein MJ, Usdin TB, Toshiyuki S, Carninci P, Prange C, Raha SS, Loquellano NA, Peters GJ, Abramson RD, Mullahy SJ, Bosak SA, McEwan PJ, McKernan KJ, Malek JA, Gunaratne PH, Richards S, Worley KC, Hale S, Garcia AM, Gay LJ, Hulyk SW, Villalon DK, Muzny DM, Sodergren EJ, Lu X, Gibbs RA, Fahey J, Helton E, Ketteman M, Madan A, Rodrigues S, Sanchez A, Whiting M, Madan A, Young AC, Shevchenko Y, Bouffard GG, Blakesley RW, Touchman JW, Green ED, Dickson MC, Rodriguez AC, Grimwood J, Schmutz J, Myers RM, Butterfield YS, Krzywinski MI, Skalska U, Smailus DE, Schnerch A, Schein JE, Jones SJ, Marra MA, Mammalian Gene Collection Program Team
Proceedings of the National Academy of Sciences of the United States of America 2002 Dec 24;99(26):16899-903

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Molecular cloning, expression, and characterization of the human mitogen-activated protein kinase p44erk1.

Charest DL, Mordret G, Harder KW, Jirik F, Pelech SL
Molecular and cellular biology 1993 Aug;13(8):4679-90

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Heterogeneous expression of four MAP kinase isoforms in human tissues.

Gonzalez FA, Raden DL, Rigby MR, Davis RJ
FEBS letters 1992 Jun 15;304(2-3):170-8

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Extracellular signal-regulated kinases in T cells: characterization of human ERK1 and ERK2 cDNAs.

Owaki H, Makar R, Boulton TG, Cobb MH, Geppert TD
Biochemical and biophysical research communications 1992 Feb 14;182(3):1416-22

Western blot

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Western blot analysis of ERK1 (arrow) using rabbit ERK1 N-term Pab.293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAPK1 gene (Lane 2)

Immunohistochemistry

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

(TOP)Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (BUTTOM)Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with ERK1 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.