BSM-33337M

antibody from Invitrogen Antibodies

Targeting: MAPK3 ERK1, p44erk1, p44mapk, PRKM3

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

BSM-33337M

Provider

Invitrogen Antibodies

Product name

ERK1/2 Monoclonal Antibody (3G4)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

3G4

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Lane 1:NIH/3T3 cell lysates probed with ERK1/2 Monoclonal Antibody, Unconjugated (bsm-33337M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Lane 1:NIH/3T3 cell lysates probed with ERK1/2 Monoclonal Antibody, Unconjugated (bsm-33337M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Lane 1:NIH/3T3 cell lysates probed with ERK1/2 Monoclonal Antibody, Unconjugated (bsm-33337M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with ERK1/2 Monoclonal Antibody, Unconjugated (bsm-33337M) at 1:2000 overnight at 4°C, DAB staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with ERK1/2 Monoclonal Antibody, Unconjugated (bsm-33337M) at 1:2000 overnight at 4°C, DAB staining.