PA1-4703

antibody from Invitrogen Antibodies

Targeting: MAPK3 ERK1, p44erk1, p44mapk, PRKM3

Western blot (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-4703

Provider

Invitrogen Antibodies

Product name

ERK1/ERK2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with mouse based on 100% sequence homology. In Western blot, PA1-4703 detects the~43 kDa ERK/MAPK protein.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 μL

Concentration

0.25 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 6 Erk1/2 expression in the B6, 129S:B6 and 129S brain. (A) Immunoblots demonstrating the expression of Erk1/2 and pErk1/2 in the hippocampus. (B,C) Bar graph illustrating Erk (1 and 2) normalized pErk1 and pErk2 expression in the whole hippocampal lysate. (D) Representative western blots illustrating the expression of pErk1 and pErk2 in hippocampal synaptosomal extracts. (E-G) Bar graphs representing Erk normalized pErk1, pErk2, and total pErk1/2 expression in hippocampal synaptosomal extracts. (H) Immunoblots illustrating prefrontal total cortical Erk1/2 expression. (I) Bar graph demonstrating a significant increase in total PFC Erk1/2 expression for the 129S:B6 and 129S mice. (J,K) Bar graph representing the expression of Erk normalized pErk1 and pErk2 expression in cortical whole lysate. (L) Schematic illustration demonstrating an increase in cortical Erk1/2 expression. [ (E-G,I,K) ; * p < 0.05, ** p < 0.01, and *** p < 0.001].