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- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- AM09379PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AM09379PU-N, RRID:AB_10758015
- Product name
- anti MAPK3 / ERK1
- Antibody type
- Monoclonal
- Antigen
- Recombinant human MAPK3 protein (1-137aa) purified from E. coli
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- AT1A2
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Cell lysates of HepG2 (35ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MAPK3 (1:500). To determine specificity of anti-human MAPK3 antibody, western blot was performed using the MAPK1 and MAPK3 recombinant proteins (50ng). Proteins were visualized using a goat anti-mouse
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis:Cell lysates of HepG2 (35 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MAPK3 (1:500). To determine specificity of anti-human MAPK3 antibody, western blot was performed using the MAPK1 and MAPK3 recombinant proteins (50ng). Proteins were visualized using a goat anti-mouse antibody.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunofluorescence of HeLa cells stained with Hoechst 3342 (Blue) for nucleus staining and monoclonal anti-human MAPK3 antibody (1:500) with Texas Red (Red).
