Antibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Immunoprecipitation [1]
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Validation data
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- Product number
- NBP2-32077 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal GABA-AR alpha 4 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Immunoprecipitation: GABA-AR alpha 4 Antibody [NBP2-32077] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from HeLa cells. Antibodies: Affinity purified rabbit anti- NBP2-32077 used for IP at 6 ug per reaction. was also immunoprecipitated by rabbit anti- BL16428. For blotting immunoprecipitated GABA-A R alpha 4 Antibody, NBP2-32077 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.