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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunohistochemistry [3]
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- Product number
- AP7949a - Provider product page
- Provider
- Abcepta
- Proper citation
- Abgent Cat#AP7949a, RRID:AB_2188409
- Product name
- SGK3 Antibody (N-term)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 1.93 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- Western blot analysis of anti-SKG3 Pab (Cat. #AP7949a) in A375 cell lysate. SGK3 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- "Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma."
- Primary Ab dilution
- 1:50~100
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- "Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with SGK3 Antibody (N-term) (Cat.#AP7949a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated."
- Primary Ab dilution
- 1:50~100
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- "IHCdetection of SGK3 protein on the paraffin sections of the WT (left) and YPC (right) mice at P0 (B and C), P3 (D and E), and P5 (F and G) skin. Positive signals were observed in the cytoplasm of the hair follicle keratinocytes, especially in hair bulb, ORS, IRS, cuticle/cortex and bulge, or sebaceous glands. Some differences between the WT and YPC, for example, the expression in bulb keratinocytes were observed at P3 and P5. Scale bar, 50."
- Primary Ab dilution
- 1:50~100
