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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- NB110-96874 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB110-96874, RRID:AB_1260806
- Product name
- Mouse Monoclonal FKBP52/FKBP4 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. Detects approx 52kDa. Heavy chain migrates close to FKBP52 on SDS PAGE.
- Reactivity
- Human, Mouse, Rat, Canine, Hamster
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: FKBP52/FKBP4 Antibody (Hi52C) [NB110-96874] - analysis of Rat Brain, Heart, Kidney, Liver, Pancreas, Skeletal muscle, Spleen, Testes, Thymus cell lysates showing detection of FKBP52 protein using Mouse Anti-FKBP52 Monoclonal Antibody, Clone Hi52C . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-FKBP52 Monoclonal Antibody at 1.5 ug/mL for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: FKBP52/FKBP4 Antibody (Hi52C) [NB110-96874] - Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-FKBP52 Monoclonal Antibody at 1.5 ug/mL for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: FKBP52/FKBP4 Antibody (Hi52C) [NB110-96874] - analysis using Mouse Anti-FKBP52 Monoclonal Antibody, Clone Hi52C . Tissue: prostate tissue (ductual epithelial cells). Species: Human. Primary Antibody: Mouse Anti-FKBP52 Monoclonal Antibody at 1:1000. Courtesy of: David F. Smith, Mayo Clinic, USA.
