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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [3]
- Flow cytometry [1]
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- Product number
- MA5-34801 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FKBP4 Recombinant Rabbit Monoclonal Antibody (JG96-30)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: 293, SK-Br-3, 293T, MCF-7, human prostate cancer tissue, human esophagus tissue, mouse brain tissue.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- JG96-30
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles, store in dark
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FKBP52 in 293 (1) and SK-Br-3 (2) cell lysate. Samples were incubated with FKBP52 monoclonal antibody (Product # MA5-34801), at a dilution of 1:2000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of FKBP52 in MCF-7 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with FKBP52 monoclonal antibody (Product # MA5-34801), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of FKBP52 in 293T cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with FKBP52 monoclonal antibody (Product # MA5-34801), followed by DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of FKBP52 in paraffin-embedded mouse brain tissue. Samples were incubated with FKBP52 monoclonal antibody (Product # MA5-34801), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of FKBP52 in paraffin-embedded human prostate cancer tissue. Samples were incubated with FKBP52 monoclonal antibody (Product # MA5-34801), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of FKBP52 in paraffin-embedded human esophagus tissue. Samples were incubated with FKBP52 monoclonal antibody (Product # MA5-34801), and followed by hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of FKBP52 in 293T cells (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Samples were incubated with FKBP52 monoclonal antibody (Product # MA5-34801) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.
