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LearnNBP2-22261
antibody from Novus Biologicals
Targeting: EXOSC3
CGI-102, hRrp-40, hRrp40p, p10, RRP40, Rrp40p
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- NBP2-22261 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP2-22261, RRID:AB_2750849
- Product name
- Rabbit Polyclonal EXOSC3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Chicken/Avian
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Deregulated Expression of Mammalian lncRNA through Loss of SPT6 Induces R-Loop Formation, Replication Stress, and Cellular Senescence.
RNAP II CTD tyrosine 1 performs diverse functions in vertebrate cells.
Nojima T, Tellier M, Foxwell J, Ribeiro de Almeida C, Tan-Wong SM, Dhir S, Dujardin G, Dhir A, Murphy S, Proudfoot NJ
Molecular cell 2018 Dec 20;72(6):970-984.e7
Molecular cell 2018 Dec 20;72(6):970-984.e7
RNAP II CTD tyrosine 1 performs diverse functions in vertebrate cells.
Hsin JP, Li W, Hoque M, Tian B, Manley JL
eLife 2014 May 8;3:e02112
eLife 2014 May 8;3:e02112
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: EXOSC3 Antibody [NBP2-22261] - Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), Jurkat (50 ug), and mouse NIH3T3 (50 ug) cells. NBP2-22261 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: EXOSC3 Antibody [NBP2-22261] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: NBP2-22261 used for IP at 6 ug/mg lysate. EXOSC3 was also immunoprecipitated by another rabbit anti-EXOSC3 antibody. For blotting immunoprecipitated EXOSC3, NBP2-22261 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
