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LearnPA5-30610
antibody from Invitrogen Antibodies
Targeting: EXOSC3
CGI-102, hRrp-40, hRrp40p, p10, RRP40, Rrp40p
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-30610 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EXOSC3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A431, Jurkat, Raji. Predicted reactivity: Mouse (86%), Rat (83%), Rhesus Monkey (97%), Chimpanzee (98%), Bovine (93%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using EXOSC3 Polyclonal Antibody (Product # PA5-30610). Sample (30 µg of whole cell lysate). Lane A: A431. Lane B: JurKat. Lane C: Raji. 12% SDS PAGE. EXOSC3 Polyclonal Antibody (Product # PA5-30610) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of EXOSC3 in paraformaldehyde-fixed HeLa cells using an EXOSC3 polyclonal antibody (Product # PA5-30610) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- EXOSC3 Polyclonal Antibody detects EXOSC3 protein at cytosol and nucleus on HeLa xenograft by immunohistochemical analysis. Sample: Paraffin-embedded HeLa xenograft. EXOSC3 Polyclonal Antibody (Product # PA5-30610) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
