OSM00017W

MCOLN1 antibody from Invitrogen Antibodies

ML4, MLIV, MST080, MSTP080, TRPM-L1, TRPML1

Western blot

Recommended by provider

Immunohistochemistry

Recommended by provider

Other assay

Supportive data in Antibodypedia

Provider product page for OSM00017W

Antibody Data

Product number

OSM00017W

Provider

Invitrogen Antibodies

Product name

TRPML1 Polyclonal Antibody

Provider product page

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

Conc. Not Determined

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.

References

MCOLN1 Promotes Proliferation and Predicts Poor Survival of Patients with Pancreatic Ductal Adenocarcinoma.
Hu ZD, Yan J, Cao KY, Yin ZQ, Xin WW, Zhang MF
Disease markers 2019;2019:9436047

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 3 In vivo animal experiments. (a) At the 14th day after injection, the tumor volume was measured every day. The growth curve showed that the tumors in the MCOLN1 group grew more slowly than controls. After inoculation of 29 days, the final tumor tissues were obtained. The tumors in the shRNA group were smaller than controls. (b, c) The expression of MCOLN1 in tumors of mice was detected by western blot or IHC. MCOLN1 expression was dramatically decreased in the shRNA group, which confirmed the effective silencing of MCOLN1 in tumors of mice in the shRNA group. ""*"" represents the significant association ( P < 0.05 for the difference was significant).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 4 The expression of MCOLN1 in tumor tissues and the associations of MCOLN1 with the prognosis of PDAC patients. (a, b) The staining of high and low expressions of MCOLN1 by IHC in PDAC tissues and paracarcinoma tissues. (c) OS and RFS rates with MCOLN1 expression in the 82 PDAC patients: shorter OS or RFS was significantly observed in high MCOLN1 expression than in low MCOLN1 expression in the 82 PDAC patients ( P < 0.05, respectively).