OSR00050W

MCOLN1 antibody from Invitrogen Antibodies

ML4, MLIV, MST080, MSTP080, TRPM-L1, TRPML1

Western blot

Supportive data in Antibodypedia

Immunohistochemistry

Supportive data in Antibodypedia

Provider product page for OSR00050W

Antibody Data

Product number

OSR00050W

Provider

Invitrogen Antibodies

Product name

TRPML1 Polyclonal Antibody

Provider product page

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

Conc. Not Determined

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on mouse kidney lysate using TRPML1 Polyclonal Antibody (Product #OSR00050W). Blocking with 0. 5% LFDM for 30 min at RT; Primary antibody used at 1:2,000 dilution incubated overnight at 4C.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis was performed in sections of rat brain using TRPML1 Polyclonal Antibody (Product #OSR00050W). The animal was perfused using Autoperfuser at a pressure of 110 mmHg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Primary antibody: dilution 1:1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis was performed in sections of rat brain using TRPML1 Polyclonal Antibody (Product #OSR00050W). The animal was perfused using Autoperfuser at a pressure of 110 mmHg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Primary antibody: dilution 1:1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis was performed in sections of rat brain using TRPML1 Polyclonal Antibody (Product #OSR00050W). The animal was perfused using Autoperfuser at a pressure of 110 mmHg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Primary antibody: dilution 1:1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis was performed in sections of rat brain using TRPML1 Polyclonal Antibody (Product #OSR00050W). The animal was perfused using Autoperfuser at a pressure of 110 mmHg with 300 ml 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Primary antibody: dilution 1:1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.