AP7932d

antibody from Abgent

Targeting: PIM1 PIM

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP7932d

Provider

Abgent

Proper citation

Abgent Cat#AP7932d, RRID:AB_2163804

Product name

PIM1 Antibody

Antibody type

Polyclonal

Antigen

Recombinant Protein

Description

Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

2 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Western blot analysis of PIM1 (arrow) using PIM1 Antibody (Cat.#AP7932d). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PIM1 gene (Lane 2) (Origene Technologies).

Primary Ab dilution

1:1000

Immunohistochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with PIM1 antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated."

Primary Ab dilution

1:10~50