Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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Validation data
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- Product number
- M01184 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-PIM1 Rabbit Monoclonal Antibody
- Antibody type
- Monoclonal
- Description
- Monoclonal antibody for PIM1 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IF, IHC, ICC, WB. Reactive species: Human, Mouse PIM1 information: Molecular Weight: 45412 MW; Subcellular Localization: Isoform 2: Cytoplasm. Nucleus; Tissue Specificity: Expressed primarily in cells of the hematopoietic and germline lineages. Isoform 1 and isoform 2 are both expressed in prostate cancer cell lines.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Antibody clone number
- ECB-16
- Vial size
- 100ug/vial
- Concentration
- 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
- Storage
- At -20°C for one year. Avoid repeated freezing and thawing.
Submitted references PIM1 polymorphism and PIM1 expression as predisposing factors of esophageal squamous cell carcinoma in the Asian population.
Wu YB, Lu D, He ZF, Jin CG
OncoTargets and therapy 2016;9:2919-25
OncoTargets and therapy 2016;9:2919-25
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human prostate carcinoma, using PIM1 Antibody (M01184)PIM1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PIM1 Antibody (M01184)overnight at 4
- Additional image