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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- Ab121877 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- Recombinant PIM1 Antibody
- Antibody type
- Recombinant
- Description
- Rabbit anti Human PIM1 Antibody, Recombinant, could be used for WB, IHC and so on.Application:WB: 1/500-1/1000IHC: 1/50-1/100Protein Function:May affect the structure or silencing of chromatin by phosphorylating HP1 gamma/CBX3. Isoform 2 promotes the G1/S transition of the cell cycle via up-regulation of CDK2 activity and phosphorylation of CDKN1B, resulting in enhanced nuclear export and proteasome-dependent degradation of CDKN1B. Isoform 2 also represses CDKN1B transcription by phosphorylating and inactivating the transcription factor FOXO3. Plays a role in signal transduction in blood cells. Contributes to both cell proliferation and survival and thus provides a selective advantage in tumorigenesis.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
AA 118-131- Vial size
- 100_l,10_l,1ml,50_l
- Concentration
- 0,3 mg/ml
- Storage
- Store at 4¡C short term (1-2 weeks). Store at -20¡C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant PIM1 Antibody (Ab121877) - Western Blot All lanes: Recombinant PIM1 Antibody (Ab121877) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 36 kDa Observed band size: 32 kDa Exposure time: 30.0 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant PIM1 Antibody (Ab121877) - IHC Immunohistochemistry analysis of paraffin-embedded Human tonsil using Recombinant PIM1 Antibody (Ab121877). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
