Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PA5-28554 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ALDH1B1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A431. Predicted reactivity: Mouse (94%), Rat (94%), Chimpanzee (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.87 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ALDH1B1 Polyclonal Antibody (Product # PA5-28554). Sample (30 µg whole cell lysate). A: A431. 7.5% SDS PAGE. ALDH1B1 Polyclonal Antibody (Product # PA5-28554) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ALDH1B1 Polyclonal Antibody detects ALDH1B1 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: ALDH1B1 protein stained by ALDH1B1 Polyclonal Antibody (Product # PA5-28554) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of ALDH1B1 was performed in paraffin-embedded mouse duodenum tissue using ALDH1B1 Polyclonal Antibody (Product # PA5-28554) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using ALDH1B1 (Product # PA5-28554) antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.