Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 12-9838-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GPNMB Monoclonal Antibody (HOST5DS), PE, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This HOST5DS monoclonal antibody recognizes human GPNMB also known as Osteoactivin, DC-HIL and HGFIN. This type-1 transmembrane glycoprotein was described independently as a bone development factor, tumor related molecule, neurokinin, and immune regulator. Expressed on macrophages and dendritic cells, GPNMB is a ligand for proteoglycan Syndecan-4 (SD-4), which is found on T cells, and is known to inhibit their activation and proliferation. Hence, surface expression of GPNMB by myeloid cells may be indicative of their tolerogenic properties. Various cancers express GPNMB, and it is suspected to help in immune evasion. Treatment with IL-10 and various immunosuppressive drugs (e.g., dexamethasone) upregulate GPNMB on macrophages and dendritic cells. THP-1 and U937 cell lines constitutively express GPNMB. GPNMB may also be secreted. The HOST5DS antibody will work on paraformaldehyde-fixed and permeabilized cells, and can be used for intracellular staining. Please, note that enzymatic tissue dissociation may reduce the amount of surface GPNMB, and thereby negatively impact the brightness of the staining. Applications Reported: This HOST5DS antibody has been reported for use in flow cytometric analysis. Applications Tested: This HOST5DS antibody has been pre-titrated and tested by flow cytometric analysis of human blood monocyte-derived macrophages. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Tissue dissociation protocols involving enzymatic digestion should be optimized to limit proteolytic degradation of GPNMB on the cell surface. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Isotype
- IgG
- Antibody clone number
- HOST5DS
- Vial size
- 100 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Glioma-associated microglia/macrophages display an expression profile different from M1 and M2 polarization and highly express Gpnmb and Spp1.
The transcription factor MITF is a critical regulator of GPNMB expression in dendritic cells.
The immune inhibitory receptor osteoactivin is upregulated in monocyte-derived dendritic cells by BCR-ABL tyrosine kinase inhibitors.
The DC-HIL/syndecan-4 pathway inhibits human allogeneic T-cell responses.
DC-HIL is a negative regulator of T lymphocyte activation.
Molecular cloning of a dendritic cell-associated transmembrane protein, DC-HIL, that promotes RGD-dependent adhesion of endothelial cells through recognition of heparan sulfate proteoglycans.
Szulzewsky F, Pelz A, Feng X, Synowitz M, Markovic D, Langmann T, Holtman IR, Wang X, Eggen BJ, Boddeke HW, Hambardzumyan D, Wolf SA, Kettenmann H
PloS one 2015;10(2):e0116644
PloS one 2015;10(2):e0116644
The transcription factor MITF is a critical regulator of GPNMB expression in dendritic cells.
Gutknecht M, Geiger J, Joas S, Dörfel D, Salih HR, Müller MR, Grünebach F, Rittig SM
Cell communication and signaling : CCS 2015 Mar 24;13:19
Cell communication and signaling : CCS 2015 Mar 24;13:19
The immune inhibitory receptor osteoactivin is upregulated in monocyte-derived dendritic cells by BCR-ABL tyrosine kinase inhibitors.
Schwarzbich MA, Gutknecht M, Salih J, Salih HR, Brossart P, Rittig SM, Grünebach F
Cancer immunology, immunotherapy : CII 2012 Feb;61(2):193-202
Cancer immunology, immunotherapy : CII 2012 Feb;61(2):193-202
The DC-HIL/syndecan-4 pathway inhibits human allogeneic T-cell responses.
Chung JS, Bonkobara M, Tomihari M, Cruz PD Jr, Ariizumi K
European journal of immunology 2009 Apr;39(4):965-74
European journal of immunology 2009 Apr;39(4):965-74
DC-HIL is a negative regulator of T lymphocyte activation.
Chung JS, Sato K, Dougherty II, Cruz PD Jr, Ariizumi K
Blood 2007 May 15;109(10):4320-7
Blood 2007 May 15;109(10):4320-7
Molecular cloning of a dendritic cell-associated transmembrane protein, DC-HIL, that promotes RGD-dependent adhesion of endothelial cells through recognition of heparan sulfate proteoglycans.
Shikano S, Bonkobara M, Zukas PK, Ariizumi K
The Journal of biological chemistry 2001 Mar 16;276(11):8125-34
The Journal of biological chemistry 2001 Mar 16;276(11):8125-34
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Normal human blood monocyte-derived macrophages were cultured in the presence of Human M-CSF Recombinant Protein (Product # 14-8789-80) and Human IL-10 Recombinant Protein (Product # 14-8109-80), and then stained with Anti-Human CD11b APC (Product # 17-0118-42) and Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (left) or Anti-Human GPNMB PE (right). Total viable cells, as determined by Fixable Viability Dye eFluor® 450 (Product # 65-0863-14), were used for analysis.
- Conjugate
- Yellow dye