Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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Validation data
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- Product number
- A02439-1 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-GPNMB/Gpnmb Picoband™ Antibody
- Antibody type
- Polyclonal
- Description
- Rabbit IgG polyclonal antibody for GPNMB/Gpnmb detection. Tested with WB, IHC-P, ICC/IF, FCM, Direct ELISA in Human;Mouse;Rat.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Downregulation of glycoprotein non-metastatic melanoma protein B prevents high glucose-induced angiogenesis in diabetic retinopathy.
Bioabsorbable nano-micelle hybridized hydrogel scaffold prevents postoperative melanoma recurrence.
Qin T, Xi X, Wu Z
Molecular and cellular biochemistry 2023 Apr;478(4):697-706
Molecular and cellular biochemistry 2023 Apr;478(4):697-706
Bioabsorbable nano-micelle hybridized hydrogel scaffold prevents postoperative melanoma recurrence.
Zhao M, Xu R, Yang Y, Tong L, Liang J, Jiang Q, Fan Y, Zhang X, Sun Y
Journal of controlled release : official journal of the Controlled Release Society 2023 Apr;356:219-231
Journal of controlled release : official journal of the Controlled Release Society 2023 Apr;356:219-231
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Supportive validation
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- Boster Biological Technology (provider)
- Main image
- Experimental details
- IHC analysis of Gpnmb using anti-Gpnmb antibody (A02439-1). Gpnmb was detected in paraffin-embedded section of human liver cancer. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Gpnmb Antibody (A02439-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
- Additional image