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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- 66926-1-IG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GPNMB Monoclonal Antibody (2B10B8)
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2B10B8
- Vial size
- 150 µL
- Concentration
- 1000 µg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Various lysates were subjected to SDS PAGE followed by western blot with 66926-1-Ig (GPNMB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66926-1-Ig (GPNMB antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66926-1-Ig (GPNMB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66926-1-Ig (GPNMB antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).
