MA1-5878
antibody from Invitrogen Antibodies
Targeting: SMN1
BCD541, GEMIN1, SMA, SMA@, SMA1, SMA2, SMA3, SMNT, TDRD16A
Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
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Validation data
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- Product number
- MA1-5878 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SMN1/SMN2 Monoclonal Antibody (2B1)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA1-5878 detects SMN in human, mouse and xenopus samples. MA1-5878 has been successfully used in Western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin), flow cytometry, and ELISA procedures. MA1-5878 detects a band of approximately 42kDa. The MA1-5878 immunogen is purified recombinant His 6 tagged-SMN protein (human).
- Reactivity
- Human, Mouse, Xenopus
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2B1
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Coupled in vitro import of U snRNPs and SMN, the spinal muscular atrophy protein.
Narayanan U, Achsel T, Lührmann R, Matera AG
Molecular cell 2004 Oct 22;16(2):223-34
Molecular cell 2004 Oct 22;16(2):223-34
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of SMN1/SMN2 was achieved by transfecting SH-SY5Y with SMN1/SMN2 specific siRNAs (Silencer® select Product # s531465, s530579). Western blot analysis (Fig. a) was performed using whole cell extracts from the SMN1/SMN2 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with SMN1/SMN2 Monoclonal Antibody (Product # MA1-5878, 1:500 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to SMN1/SMN2.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-SMN1/SMN2 Monoclonal Antibody (Product # MA1-5878) and a 37 kDa band corresponding to SMN1/SMN2 was observed across all the cell lines tested. Whole cell extracts (30 µg lysate) of SH-SY5Y (Lane 1), PC-3 (Lane 2), Caco-2 (Lane 3), HeLa (Lane 4), Hep G2 (Lane 5) and NK-92 (Lane 6) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of SMN using monoclonal antibody (Product # MA1-5878).