MA1-5878

antibody from Invitrogen Antibodies

Targeting: SMN1 BCD541, GEMIN1, SMA, SMA@, SMA1, SMA2, SMA3, SMNT, TDRD16A

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Flow cytometry (Recommended by provider)

Antibody Data

Product number

MA1-5878

Provider

Invitrogen Antibodies

Product name

SMN1/SMN2 Monoclonal Antibody (2B1)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA1-5878 detects SMN in human, mouse and xenopus samples. MA1-5878 has been successfully used in Western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin), flow cytometry, and ELISA procedures. MA1-5878 detects a band of approximately 42kDa. The MA1-5878 immunogen is purified recombinant His 6 tagged-SMN protein (human).

Reactivity

Human, Mouse, Xenopus

Host

Mouse

Isotype

IgG

Antibody clone number

2B1

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Coupled in vitro import of U snRNPs and SMN, the spinal muscular atrophy protein.

Narayanan U, Achsel T, Lührmann R, Matera AG
Molecular cell 2004 Oct 22;16(2):223-34

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Knockdown of SMN1/SMN2 was achieved by transfecting SH-SY5Y with SMN1/SMN2 specific siRNAs (Silencer® select Product # s531465, s530579). Western blot analysis (Fig. a) was performed using whole cell extracts from the SMN1/SMN2 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with SMN1/SMN2 Monoclonal Antibody (Product # MA1-5878, 1:500 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to SMN1/SMN2.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-SMN1/SMN2 Monoclonal Antibody (Product # MA1-5878) and a 37 kDa band corresponding to SMN1/SMN2 was observed across all the cell lines tested. Whole cell extracts (30 µg lysate) of SH-SY5Y (Lane 1), PC-3 (Lane 2), Caco-2 (Lane 3), HeLa (Lane 4), Hep G2 (Lane 5) and NK-92 (Lane 6) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of SMN using monoclonal antibody (Product # MA1-5878).