720207

antibody from Invitrogen Antibodies

Targeting: SMN1 BCD541, GEMIN1, SMA, SMA@, SMA1, SMA2, SMA3, SMNT, TDRD16A

Antibody data

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Validation data

Product number: 720207 - Provider product page
Provider: Invitrogen Antibodies
Product name: SMN1 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Other
Description: These Polyclonal antibodies are of rabbit origin developed by immunizing animals with proteins or peptides. The polyclonal antibody is purified by affinity purification from the rabbit sera generated after immunizing the rabbits with a specific type of protein or peptide. The purified antibody is tested for its functionality in various relevant research applications. The antibody is developed for Research Use Only and is non-hazardous or non-infectious in nature.
Concentration: 0.5 mg/mL

SMN1 protein structure - 720207 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of SMN was achieved by transfecting SH-SY5Y with SMN specific siRNAs (Silencer® select Product # s531465, s530579). Western blot analysis (Fig. a) was performed using Whole cell extracts from the SMN knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with SMN Polyclonal Antibody (Product # 720207, 1:1000 ) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to SMN.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-SMN Polyclonal Antibody (Product # 720207) and a 35 kDa band corresponding to SMN was observed across cell lines tested. Whole cell extracts (30 µg lysate) of SH-SY5Y (Lane 1), PC-3 (Lane 2), Caco-2 (Lane 3), HeLa (Lane 4), Hep G2 (Lane 5) or U-87 MG (Lane 6) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on Whole cell extracts (30 µg lysate) of SH-SY 5Y (Lane 1), PC-3 (Lane 2), Caco-2 (Lane 3), HeLa (Lane 4), Jurkat (Lane 5), A-431 (Lane 6), COS-7 (Lane 7) and Raji (Lane 8). The blots were probed with Anti-SMN1 Rabbit Polyclonal Antibody (Product # 720207, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 39 kDa band corresponding to SMN1 was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex®NuPAGE®4-12% Bis-Tris gel (Product # NP0321BOX), XCell SureLock Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).