60154-1-IG
antibody from Invitrogen Antibodies
Targeting: SMN1
BCD541, GEMIN1, SMA, SMA@, SMA1, SMA2, SMA3, SMNT, TDRD16A
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [7]
- Flow cytometry [1]
- Other assay [1]
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Validation data
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- Product number
- 60154-1-IG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SMN Monoclonal Antibody (2C6D9)
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2C6D9
- Vial size
- 150 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Raji cells were subjected to SDS PAGE followed by western blot with 60154-1-Ig (SMN (Human-Specific) antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 60154-1-Ig (SMN (Human-Specific) antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- A375, HEK-293, and Jurkat cells were subjected to SDS PAGE followed by western blot with 60154-1-IG (SMN (Human-Specific) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells using 60154-1-IG (SMN (Human-Specific) antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IGG.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney using 60154-1-Ig (SMN (Human-Specific) antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney using 60154-1-Ig (SMN (Human-Specific) antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human heart using 60154-1-Ig (SMN (Human-Specific) antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human heart using 60154-1-Ig (SMN (Human-Specific) antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver using 60154-1-Ig (SMN (Human-Specific) antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver using 60154-1-Ig (SMN (Human-Specific) antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human brain using 60154-1-IG (SMN (Human-Specific) antibody) at dilution of 1:50 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 Jurkat cells were stained with 0.20ug SMN (Human-Specific) antibody (60154-1-IG, red) and control antibody (blue). Fixed with 90% MeOH.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-SMN (Human-Specific) (IP:60154-1-IG, 4ug; Detection:60154-1-IG 1:500) with HEK-293 cells lysate 2440ug.