MA5-15858

BCD541, GEMIN1, SMA, SMA@, SMA1, SMA2, SMA3, SMNT, TDRD16A

Antibody data

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Validation data

Product number: MA5-15858
Provider: Invitrogen Antibodies
Product name: Anti-SMN1 Monoclonal Antibody (5H1)
Provider product page: Invitrogen Antibodies - MA5-15858
Antibody type: Monoclonal
Antigen: Recombinant protein fragment
Description: MA5-15858 targets SMN1 in IF, IHC, and WB applications and shows reactivity with Human and Non-human primate samples. The MA5-15858 immunogen is purified recombinant fragment of human SMN1 expressed in E. Coli. . MA5-15858 detects SMN1 which has a predicted molecular weight of approximately 39kDa.
Reactivity: Human
Host: Mouse
Isotype: IgG
Antibody clone number: 5H1
Vial size: 100 µL
Concentration: Conc. Not Determined
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Provider	Type	Product Number
- No reagents -

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of SMN1 using SMN1 monoclonal antibody (Product # MA5-15858) in RAJI (1), COS-7 (2), Jurkat (3), K562 (4), HeLa (5) and HepG2 (6) cell lysate.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of SMN1 was achieved by transfecting SH-SY5Y with SMN1 specific siRNAs (Silencer® select Product # s531465, s530579). Western blot analysis (Fig. a) was performed using whole cell extracts from the SMN1 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with SMN1 Monoclonal Antibody (Product # MA5-15858, 1:2000 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to SMN1.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-SMN1 Monoclonal Antibody (Product # MA5-15858) and a 37 kDa band corresponding to SMN1 was observed across all the cell lines tested along with an uncharacterized band (*) at ~70 kDa. Whole cell extracts (30 µg lysate) of SH-SY5Y (Lane 1), PC-3 (Lane 2), Caco-2 (Lane 3), HeLa (Lane 4), Hep G2 (Lane 5) and NK-92 (Lane 6) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).