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LearnA304-815A
antibody from Invitrogen Antibodies
Targeting: ARHGDIB
GDIA2, GDID4, Ly-GDI, RAP1GN1, RhoGDI2
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- A304-815A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ARHGDIB/D4-GDI/RhoGDI2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human ARHGDIB/D4-GDI/RhoGDI2 by western blot. Samples: Whole cell lysate (10 µg) from Jurkat, HEK293T, CCRF-CEM, HeLa, and HL-60 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ARHGDIB/D4-GDI/RhoGDI2 antibody (Product # A304-815A lot 2) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 3 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human ARHGDIB/D4-GDI by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ARHGDIB/D4-GDI antibody A304-815A (lot A304-815A-1) used for IP at 6 µg per reaction. ARHGDIB/D4-GDI was also immunoprecipitated by rabbit anti-ARHGDIB/D4-GDI antibody BL18679. For blotting immunoprecipitated ARHGDIB/D4-GDI, A304-815A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human ARHGDIB/D4-GDI by western blot. Samples: Whole cell lysate (50 µg) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ARHGDIB/D4-GDI antibody A304-815A (lot A304-815A-1) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human ARHGDIB/D4-GDI by immunohistochemistry. Sample: FFPE section of human breast carcinoma inflammatory cell infiltrate. Antibody: Affinity purified rabbit anti-ARHGDIB/D4-GDI (A304-815A; lot 2) used at a dilution of 1:5,000 0.2 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human ARHGDIB/D4-GDI by immunohistochemistry. Sample: FFPE section of human stomach carcinoma inflammatory cell infiltrate. Antibody: Affinity purified rabbit anti- ARHGDIB/D4-GDI (Cat. No. A304-815A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human ARHGDIB/D4-GDI by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ARHGDIB/D4-GDI antibody (Product # A304-815A lot 2) used for IP at 6 µg per reaction. ARHGDIB/D4-GDI was also immunoprecipitated by a previous lot of this antibody (A304-815A lot 1) and rabbit anti-ARHGDIB/D4-GDI antibody. For blotting immunoprecipitated ARHGDIB/D4-GDI (Product # A304-815A) was used at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 3 seconds.
