Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- NBP1-71838 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-71838, RRID:AB_11037795
- Product name
- Rabbit Polyclonal NAB1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Type I IFNs downregulate myeloid cell IFN-γ receptor by inducing recruitment of an early growth response 3/NGFI-A binding protein 1 complex that silences ifngr1 transcription.
Kearney SJ, Delgado C, Eshleman EM, Hill KK, O'Connor BP, Lenz LL
Journal of immunology (Baltimore, Md. : 1950) 2013 Sep 15;191(6):3384-92
Journal of immunology (Baltimore, Md. : 1950) 2013 Sep 15;191(6):3384-92
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: NAB1 Antibody [NBP1-71838] - Simple Western lane view shows a specific band for NAB1 in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NAB1 Antibody [NBP1-71838] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T, 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Affinity purified rabbit anti-NAB1 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 6 mcg/mg lysate. NAB1 was also immunoprecipitated by rabbit anti-NAB1 antibody NBP1-71837, which recognizes an upstream epitope.