15131-1-AP

antibody from Proteintech Group

Targeting: ATG4B APG4B, AUTL1, DKFZp586D1822, KIAA0943

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

15131-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#15131-1-AP, RRID:AB_2064405

Product name

ATG4B antibody

Antibody type

Polyclonal

Description

KD/KO validated ATG4B antibody (Cat. #15131-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IHC, IP, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Ginsenoside Rg3 Mitigates LPS-Induced Injury in Human Bronchial Epithelial Cells by Restoring Autophagic Flux and Inhibiting the TLR4/NF-κB-Mediated Inflammatory Response.

Tao X, Liu L, Gu X, Jia B, Yu Q, Wei Y, Zhang W, Zhou J
Journal of inflammation research 2026;19:555053

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Hexavalent chromium induced autophagy-dependent mTOR expression mediated by upregulation of HMGA2.

Li L, Dan M, Li S, Cui X, Bao S, Luo S, Cao J
Toxicology 2026 Feb;520:154368

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TDP-43 dysfunction compromises UPF1-dependent mRNA metabolism in ALS.

Alessandrini F, Wright M, Kurosaki T, Perloff OS, Ngo M, Kofler JK, Donnelly CJ, Maquat LE, Kiskinis E
Neuron 2026 Feb 18;114(4):640-660.e10

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RHOD mediates ATG9A trafficking to promote autophagosome formation during autophagy in cancer.

Wang S, Ren J, Chi J, Guan Y, Zheng R, Wang J, Liu X, Huang H
Autophagy 2025 Sep;21(9):1976-1994

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Rab27a regulates the transport of influenza virus membrane proteins to the plasma membrane.

Chen T, Ouyang A, Zou J, Feng Y, Wu C, Jiang M, Tu S, Ding L, Cheng Y, Hu W, Sheng W, Li Y, Jin M, Chen H, Zhou H
Nature communications 2025 Jul 8;16(1):6271

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Targeting STK26 and ATG4B: miR-22-3p as a modulator of autophagy and tumor progression in HCC.

Li K, Bai Y, Wang J, Ren L, Mo A, Liu R, Wang Y, Zhou F, Pei W, Shi X
Translational oncology 2025 Jan;51:102214

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Targeting chaperone-mediated autophagy inhibits properties of glioblastoma stem cells and restores anti-tumor immunity.

Li Y, Sheng M, Li W, Liu S, Wang B, Liu B, Luo M, Zhou X, Xia Q, Hong S, Zheng Z, Cheng SY, Jiang X, Li J, Huang T
Nature communications 2025 Dec 13;17(1):440

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A biomimetic multimodal nanoplatform combining neutrophil-coated two-dimensional metalloporphyrinic framework nanosheet and exendin-4 to treat obesity-related osteoporosis.

Yang Q, Liu J, Liu Y, Liu S, Wei X, Yang Y, Zhang W, Zhang S, Zhang M, Liu B, Wang X, Zhu D
Materials today. Bio 2025 Aug;33:102009

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Targeting GABARAPL1/HIF-2a axis to induce tumor cell apoptosis in nasopharyngeal carcinoma.

Huang X, Zhou L, Chen J, Zhang S
Iranian journal of basic medical sciences 2024;27(2):157-164

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Silencing of tropomodulin 1 inhibits acute myeloid leukemia cell proliferation and tumor growth by elevating karyopherin alpha 2-mediated autophagy.

Xia Y, Wang D, Zhao H, Meng T, Jiang Q, Pan Z, Wang G, An T, Li B, Bi S, Wang H, Lu J, Liu H, Lin H, Lin C, Zheng Q, Li D
Pharmacological research 2024 Sep;207:107327

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UBE3C tunes autophagy via ATG4B ubiquitination.

Sun C, Chen Y, Gu Q, Fu Y, Wang Y, Liu C, Xie H, Liao Y, Zheng Z, Liu P, Li M
Autophagy 2024 Mar;20(3):645-658

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p53-dependent HIF-1α /autophagy mediated glycolysis to support Cr(VI)-induced cell growth and cell migration.

Yang Y, Song B, Guo M, Gao J, Jiang L, Li Q, Liu Y, Cao J
Ecotoxicology and environmental safety 2024 Mar 1;272:116076

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Lopinavir enhances anoikis by remodeling autophagy in a circRNA-dependent manner.

Wu Y, Chen Y, Yan X, Dai X, Liao Y, Yuan J, Wang L, Liu D, Niu D, Sun L, Chen L, Zhang Y, Xiang L, Chen A, Li S, Xiang W, Ni Z, Chen M, He F, Yang M, Lian J
Autophagy 2024 Jul;20(7):1651-1672

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ATF4/PHGDH mediates the effects of ER stress on cadmium-induced autophagy and glycolysis.

Yang Y, Li S, Yang Y, Li Q, Liu Y, Cao J
Toxicology 2024 Dec;509:153976

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ATG4B antagonizes antiviral immunity by GABARAP-directed autophagic degradation of TBK1.

Xie W, Zhang C, Wang Z, Chen H, Gu T, Zhou T, Wu Y, Xia F, Li M, Wang J, Jiao R, Cui J, Jin S
Autophagy 2023 Nov;19(11):2853-2868

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Inhibition of ATF4-mediated elevation of both autophagy and AKT/mTOR was involved in antitumorigenic activity of curcumin.

Yang J, Hasenbilige, Bao S, Luo S, Jiang L, Li Q, Kong Y, Cao J
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2023 Mar;173:113609

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m(6)A reader HNRNPA2B1 destabilization of ATG4B regulates autophagic activity, proliferation and olaparib sensitivity in breast cancer.

Zheng R, Yu Y, Lv L, Zhang Y, Deng H, Li J, Zhang B
Experimental cell research 2023 Mar 1;424(1):113487

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Hydrogen sulfide inhibits human T-cell leukemia virus type-1 (HTLV-1) protein expression via regulation of ATG4B.

Liu H, Sun J, Cheng X, Duan L, Guo S, Zhang Z, Wan J, Wang C, Zhi X, Yuan L, Wang H
Journal of medical virology 2023 Jan;95(1):e28176

---

Comprehensive analysis of autophagic functions of WIPI family proteins and their implications for the pathogenesis of β-propeller associated neurodegeneration.

Shimizu T, Tamura N, Nishimura T, Saito C, Yamamoto H, Mizushima N
Human molecular genetics 2023 Aug 7;32(16):2623-2637

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Stratification of diabetic kidney diseases via data-independent acquisition proteomics-based analysis of human kidney tissue specimens.

Huang Q, Fei X, Zhong Z, Zhou J, Gong J, Chen Y, Li Y, Wu X
Frontiers in endocrinology 2022;13:995362

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Phospholipid peroxidation inhibits autophagy via stimulating the delipidation of oxidized LC3-PE.

Li W, Luo LX, Zhou QQ, Gong HB, Fu YY, Yan CY, Li E, Sun J, Luo Z, Ding ZJ, Zhang QY, Mu HL, Cao YF, Ouyang SH, Kurihara H, Li YF, Sun WY, Li M, He RR
Redox biology 2022 Sep;55:102421

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Curcumin attenuates Cr (VI)-induced cell growth and migration by targeting autophagy-dependent reprogrammed metabolism.

Dlamini MB, Bao S, Gao Z, Mei J, Ge H, Jiang L, Geng C, Li Q, Shi X, Liu Y, Cao J
Journal of biochemical and molecular toxicology 2022 Nov;36(11):e23193

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Downregulation of beclin 1 restores arsenite-induced impaired autophagic flux by improving the lysosomal function in the brain.

Zhou H, Ling H, Li Y, Jiang X, Cheng S, Zubeir GM, Xia Y, Qin X, Zhang J, Zou Z, Chen C
Ecotoxicology and environmental safety 2022 Jan 1;229:113066

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Targeting autophagy peptidase ATG4B with a novel natural product inhibitor Azalomycin F4a for advanced gastric cancer.

Zhong L, Yang B, Zhang Z, Wang J, Wang X, Guo Y, Huang W, Wang Q, Cai G, Xia F, Zhou S, Ma S, Nie Y, Lei J, Li M, Liu P, Deng W, Liu Y, Han F, Wang J
Cell death & disease 2022 Feb 18;13(2):161

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Discovery and mechanism studies of a novel ATG4B inhibitor Ebselen by drug repurposing and its anti-colorectal cancer effects in mice.

Xie H, Qiang P, Wang Y, Xia F, Liu P, Li M
Cell & bioscience 2022 Dec 21;12(1):206

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LncRNA CRNDE Promotes ATG4B-Mediated Autophagy and Alleviates the Sensitivity of Sorafenib in Hepatocellular Carcinoma Cells.

Chen L, Sun L, Dai X, Li T, Yan X, Zhang Y, Xiao H, Shen X, Huang G, Xiang W, Zhang Y, Tan D, Yang S, Nie Y, Huang X, Lian J, He F
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Hydrogen sulfide ameliorates doxorubicin‑induced myocardial fibrosis in rats via the PI3K/AKT/mTOR pathway.

Nie L, Liu M, Chen J, Wu Q, Li Y, Yi J, Zheng X, Zhang J, Chu C, Yang J
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Notoginsenoside R1 Protects Against the Acrylamide-Induced Neurotoxicity via Upregulating Trx-1-Mediated ITGAV Expression: Involvement of Autophagy.

Wang W, Huang L, Thomas ER, Hu Y, Zeng F, Li X
Frontiers in pharmacology 2020;11:559046

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Inhibition of MGMT-mediated autophagy suppression decreases cisplatin chemosensitivity in gastric cancer.

Lei Y, Tang L, Hu J, Wang S, Liu Y, Yang M, Zhang J, Tang B
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2020 May;125:109896

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New Anti-Cancer Strategy to Suppress Colorectal Cancer Growth Through Inhibition of ATG4B and Lysosome Function.

Fu Y, Gu Q, Luo L, Xu J, Luo Y, Xia F, Han F, Hong L, Yin XM, Huang Z, Li M
Cancers 2020 Jun 10;12(6)

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The exosome-like vesicles from osteoarthritic chondrocyte enhanced mature IL-1β production of macrophages and aggravated synovitis in osteoarthritis.

Ni Z, Kuang L, Chen H, Xie Y, Zhang B, Ouyang J, Wu J, Zhou S, Chen L, Su N, Tan Q, Luo X, Chen B, Chen S, Yin L, Huang H, Du X, Chen L
Cell death & disease 2019 Jul 8;10(7):522

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The interaction of Atg4B and Bcl-2 plays an important role in Cd-induced crosstalk between apoptosis and autophagy through disassociation of Bcl-2-Beclin1 in A549 cells.

Li Z, Li Q, Lv W, Jiang L, Geng C, Yao X, Shi X, Liu Y, Cao J
Free radical biology & medicine 2019 Jan;130:576-591

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RUBCN/rubicon and EGFR regulate lysosomal degradative processes in the retinal pigment epithelium (RPE) of the eye.

Muniz-Feliciano L, Doggett TA, Zhou Z, Ferguson TA
Autophagy 2017;13(12):2072-2085

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HSF1 upregulates ATG4B expression and enhances epirubicin-induced protective autophagy in hepatocellular carcinoma cells.

Zhang N, Wu Y, Lyu X, Li B, Yan X, Xiong H, Li X, Huang G, Zeng Y, Zhang Y, Lian J, Ni Z, He F
Cancer letters 2017 Nov 28;409:81-90

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p62/sequestosome 1 binds to TDP-43 in brains with frontotemporal lobar degeneration with TDP-43 inclusions.

Tanji K, Zhang HX, Mori F, Kakita A, Takahashi H, Wakabayashi K
Journal of neuroscience research 2012 Oct;90(10):2034-42

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

human brain tissue were subjected to SDS PAGE followed by western blot with 15131-1-AP(ATG4B antibody) at dilution of 1:800

Sample type

tissue

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human pancreas cancer using 15131-1-AP(ATG4B antibody) at dilution of 1:50 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The ATG4B antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human ATG4B. This antibody recognizes human,mouse,rat antigen. The ATG4B antibody has been validated for the following applications: ELISA, WB, IHC analysis.

Appendix

15131-1-AP.pdf

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