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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- ARG54818 - Provider product page
- Provider
- Arigo
- Product name
- anti-ATG4B antibody
- Antibody type
- Polyclonal
- Antigen
- KLH-conjugated synthetic peptide around aa. 358-390 (C-terminus) of Human ATG4B.
- Description
- This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µl
- Storage
- For continuous use, store undiluted antibody at 2-8°C for up to 6 months. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 2 µg of 293 cell line lysates transiently transfected with the ATG4B gene stained with ARG54818 anti-ATG4B antibody.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: HeLa cell lysate stained with ARG54818 anti-ATG4B antibody.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: U251 cells stained with ARG54818 anti-ATG4B antibody (green) at 1:100 dilution, 2 h at room temperature. U251 cells were treated with Chloroquine (50 µM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with ARG54818 anti-ATG4B antibody. Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 5 min). ATG4B immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: formalin-fixed and paraffin-embedded human cancer tissue stained with ARG54818 anti-ATG4B antibody.
