TA810189

antibody from OriGene

Targeting: ATG4B APG4B, AUTL1, DKFZp586D1822, KIAA0943

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA810189

Provider

OriGene

Product name

ATG4B mouse monoclonal antibody,clone OTI1A3

Antibody type

Monoclonal

Description

ATG4B mouse monoclonal antibody,clone OTI1A3

Host

Mouse

Conjugate

Unconjugated

Epitope

ATG4B

Isotype

IgG

Antibody clone number

OTI1A3

Vial size

100 µl

Concentration

1.00mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATG4B (RC216453, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATG4B.(1:2000)

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 7 different cell lines by using anti-ATG4B monoclonal antibody . (1:500)

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ATG4B mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA810189)(1:2000)

Validation comment

IHC