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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- PA5-28430 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TRIP15 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: A431, H1299, HeLa, HepG2. Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (100%), Pig (100%), Chicken (100%), Rhesus Monkey (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.76 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using TRIP15 Polyclonal Antibody (Product # PA5-28430). Sample (30 µg of whole cell lysate). Lane A: A431. Lane B: H1299. Lane C: Hela. 10% SDS PAGE. TRIP15antibody . TRIP15 Polyclonal Antibody (Product # PA5-28430) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of TRIP15 in paraformaldehyde-fixed HeLa cells using a TRIP15 polyclonal antibody (Product # PA5-28430) at a 1:500 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Mahlavu xenograft, using COPS2 (Product # PA5-28430) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TRIP15 Polyclonal Antibody detects TRIP15 protein at cytoplasm and nucleus on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. TRIP15 Polyclonal Antibody (Product # PA5-28430) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
